ArticleBiophysical journal2006
Thermodynamic comparison of the interactions of cholesterol with unsaturated phospholipid and sphingomyelins.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Chemical potential measurements constrain models of cholesterol-phosphatidylcholine interactions.Biophysical journal · 2023Article
- Distribution of cholesterol in asymmetric membranes driven by composition and differential stress.Biophysical journal · 2022Article
- Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.Scientific reports · 2020Article
- Bicelles Rich in both Sphingolipids and Cholesterol and Their Use in Studies of Membrane Proteins.Journal of the American Chemical Society · 2020Article
- Impact of Acyl Chain Mismatch on the Formation and Properties of Sphingomyelin-Cholesterol Domains.Biophysical journal · 2019Article
- Kiss and Run Asymmetric Vesicles to Investigate Coupling.Biophysical journal · 2019Article
- The Affinity of Sterols for Different Phospholipid Classes and Its Impact on Lateral Segregation.Biophysical journal · 2019Article
- Preparation of Asymmetric Liposomes Using a Phosphatidylserine Decarboxylase.Biophysical journal · 2018Article
- Membrane properties of hydroxycholesterols related to the brain cholesterol metabolism.Beilstein journal of organic chemistry · 2017Article
- cDICE method produces giant lipid vesicles under physiological conditions of charged lipids and ionic solutions.Soft matter · 2016Article
- The Affinity of Cholesterol for Different Phospholipids Affects Lateral Segregation in Bilayers.Biophysical journal · 2016Article
- Review
- Area per lipid and cholesterol interactions in membranes from separated local-field (13)C NMR spectroscopy.Biophysical journal · 2014Article
- Single lipid extraction: the anchoring strength of cholesterol in liquid-ordered and liquid-disordered phases.Biophysical journal · 2014Article
- Computational microscopy of cyclodextrin mediated cholesterol extraction from lipid model membranes.Scientific reports · 2013Article
- Importance of the sphingoid base length for the membrane properties of ceramides.Biophysical journal · 2012Article
- Monte Carlo simulation of protein-induced lipid demixing in a membrane with interactions derived from experiment.Biophysical journal · 2011Article
- Lipid-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2011Review
- Sterols have higher affinity for sphingomyelin than for phosphatidylcholine bilayers even at equal acyl-chain order.Biophysical journal · 2011Article
- An NMR database for simulations of membrane dynamics.Biochimica et biophysica acta · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comparative analysis of the interaction of cholesterol (Chol) with palmitoyl-oleoyl-phosphatidylcholine (POPC) and sphingomyelins (SM) was performed in largely homogeneous, fluid-phase membranes at 50 degrees C. To this end, three independent assays for isothermal titration calorimetry were applied to POPC/SM/Chol mixtures. Cholesterol is solubilized by randomly methylated-beta-cyclodextrin and the uptake of Chol into (or release from) large unilamellar vesicles is measured. The affinity of Chol to a POPC/SM (1:1) membrane with 30 mol % Chol is approximately two times higher than to POPC alone; extrapolation to pure SM yields an affinity ratio of R(K) approximately 5. Bringing Chol in contact with SM is highly exothermic (-7 kJ/mol for POPC/SM (1:1), and -13 kJ/mol extrapolated to pure SM, both compared to POPC). No pronounced differences were observed between egg, bovine brain, and palmitoyl SM. With decreasing Chol content, R(K) increases and deltaH becomes more exothermic, suggesting a trend toward superlattice formation. That SM/Chol-interactions are enthalpically favorable implies that the preference of Chol for SM increases upon cooling and can induce domain formation below a certain temperature. The enthalpy gain is partially compensated by a loss in entropy in accordance with the concept of Chol-induced chain ordering, which improves intermolecular interactions (van der Waals, H-bond) but reduces conformational and motional freedom. The ability of cyclodextrin to extract sphingomyelin from membranes is twofold-weaker than for POPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.