ArticlePlanta2006
Transgenic Medicago truncatula plants obtained from Agrobacterium tumefaciens -transformed roots and Agrobacterium rhizogenes-transformed hairy roots.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 97 citations in OpenAlex.
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Genetic transformation ofFrontiers in plant science · 2025Article
- Article
- Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system.Scientific reports · 2023Article
- Systematic approaches to C-lignin engineering in Medicago truncatula.Biotechnology for biofuels and bioproducts · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Challenges and potentials of new breeding techniques inFrontiers in plant science · 2023Review
- An Optimized Transformation System and Functional Test ofInternational journal of molecular sciences · 2021Article
- Optimization of Tissue Culturing and Genetic Transformation Protocol forFrontiers in plant science · 2021Article
- Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp.Nature communications · 2020Article
- Efficient Generation of CRISPR/Cas9-Mediated Homozygous/BiallelicFrontiers in plant science · 2020Article
- Genome-wide characterization of SPL family in Medicago truncatula reveals the novel roles of miR156/SPL module in spiky pod development.BMC genomics · 2019Article
- Article
- Comparison of efficiency and time to regeneration ofPlant methods · 2019Article
- From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa.Plant biotechnology journal · 2018Article
- Specialized Plant Metabolism Characteristics and Impact on Target Molecule Biotechnological Production.Molecular biotechnology · 2018Review
- Genotype-independentPlant methods · 2018Article
- Field Guide to Plant Model Systems.Cell · 2016Review
- Identification and Expression Analysis of BURP Domain-Containing Genes in Medicago truncatula.Frontiers in plant science · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Medicago truncatula, barrel medic, is a forage crop that has been developed into a model legume. The development of new transformation methods is important for functional genomic studies in this species. Based on Agrobacterium tumefaciens-mediated transformation of root explants, we developed an effective system for producing M. truncatula (genotype R108) transgenic plants. Among the four A. tumefaciens strains (AGL1, C58C1, EHA105 and LBA4404) tested, EHA105 and AGL1 were most effective in regenerating transgenics. Callus induction frequency from root explants was 69.8%, and plantlet/shoot regeneration frequency was 41.3% when EHA105 was used. Transgenic nature of the regenerated plants was confirmed by PCR and Southern hybridization analyses. Progeny analysis revealed stable Mendelian meiotic transmission of transgenes. Because M. truncatula is particularly useful for the study of root endosymbiotic associations, we further developed a plant regeneration system from A. rhizogenes-transformed hairy roots of M. truncatula. Fertile true transgenic plants were regenerated from the hairy roots, thus allowing the assessment of gene functions at the whole plant level. Segregation analysis revealed that the hairy root genes could be segregated out in the progenies. By coupling A. rhizogenes-mediated hairy root transformation and the regeneration system reported here, once potential genes of interest are identified, the transformed hairy roots carrying such genes could be directly regenerated into plants for more detailed characterization of the genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.