ArticlePlant cell reports2006
Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f., as revealed by ISSR and RAPD markers.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 86 citations in OpenAlex.
- Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally.International journal of molecular sciences · 2023Article
- Fermented Sprouts ofPharmaceutics · 2023Article
- Histological and molecular insights in to in vitro regeneration pattern of Xanthosoma sagittifolium.Scientific reports · 2023Article
- In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants.BMC plant biology · 2023Article
- Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers.Scientific reports · 2022Article
- Cytokinin-Based Tissue Cultures for Stable Medicinal Plant Production: Regeneration and Phytochemical Profiling ofBiomolecules · 2021Article
- Supplementary Light Source Affects the Growth and Development ofInternational journal of molecular sciences · 2018Article
- Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers.PloS one · 2017Article
- DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.).Journal of biological research (Thessalonike, Greece) · 2016Article
- Molecular authentication of Tetrastigma hemsleyanum from its adulterant species using ISSR, CAPS, and ITS2 barcode.Molecular biology reports · 2016Article
- Review
- The genus Codonopsis (Campanulaceae): a review of phytochemistry, bioactivity and quality control.Journal of natural medicines · 2015Article
- In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Article
- Bak is a key molecule in apoptosis induced by methanol extracts of Codonopsis lanceolata and Tricholoma matsutake in HSC-2 human oral cancer cells.Oncology letters · 2012Article
- Antilipogenic and anti-inflammatory activities of Codonopsis lanceolata in mice hepatic tissues after chronic ethanol feeding.Journal of biomedicine & biotechnology · 2012Article
- In silico gene expression analysis in Codonopsis lanceolata root.Molecular biology reports · 2011Article
- Somatic embryogenesis in peach palm using the thin cell layer technique: induction, morpho-histological aspects and AFLP analysis of somaclonal variation.Annals of botany · 2007Article
- DNA methods for identification of Chinese medicinal materials.Chinese medicine · 2007Article
- Tissue culture-induced locus-specific alteration in DNA methylation and its correlation with genetic variation in Codonopsis lanceolata Benth. et Hook. f.Plant cell reports · 2007Article
- Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers, and by pairwise sequence analysis.Journal of applied genetics · 2007Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Codonopsis lanceolata Benth. et Hook. f., commonly known as bonnet bellflower, is a high-valued herb medicine and vegetable. In this study, a large number of plants were regenerated via organogenesis from immature seed-derived calli in C. lanceolata by a simple and efficient method. Compared with the mother donor plant, the regenerated plants did not exhibit visible phenotypic variations in six major morphological traits examined at the stage of one-season-maturity under field conditions. To gain insight into the genomic stability of these regenerated plants, 63 individuals were randomly tagged among a population of more than 2,000 regenerants, and were compared with the single mother donor plant by two molecular markers, the inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD). Apparent genomic variation was detected in the 63 regenerants, whereas preexisting heterozygosiy in the donor plant was deemed minimal by testing 30 seedlings germinated from selfed seeds of the same donor plant. The percentages of polymorphic bands (PPB) in the ISSR and RAPD analysis were respectively 15.7 and 24.9% for the 63 regenerated plants. Cluster analysis indicates that the genetic similarity values calculated on the basis of RAPD and ISSR data among the 64 plants (63 regenerated and one donor) were respectively 0.894 and 0.933, which allow classification of the plants into distinct groups. Nineteen randomly isolated bands underlying the changed RAPD or ISSR patterns were sequenced, and three of them showed significant homology to known-function genes. Detailed pairwise sequence comparison at one locus between the donor plant and a regenerant revealed that insertion of two short (24 and 19 bp) stretches of nucleotides in the regenerated plant relative to the donor plant occurred in an apparently stochastic manner.
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