ArticleMolecular biology of the cell2006
Myosin VI stabilizes an actin network during Drosophila spermatid individualization.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 91 citations in OpenAlex.
- Review
- Myosin VI is expressed in developing ovarian follicles inFrontiers in cell and developmental biology · 2025Article
- Hydrogen/Deuterium Exchange Mass Spectrometry Provides Insights into the Role of Drosophila Testis-Specific Myosin VI Light Chain AndroCaM.Biochemistry · 2024Article
- Spem2, a novel testis-enriched gene, is required for spermiogenesis and fertilization in mice.Cellular and molecular life sciences : CMLS · 2024Article
- Myosin VI powers self-organization of branched contractile actin network.Biochemical and biophysical research communications · 2024Article
- Lagging X chromatids specify the orientation of asymmetric organelle partitioning in XX spermatocytes of Auanema rhodensis.Genetics · 2022Article
- PFTAIRE Kinase L63 Interactor 1A (Pif1A Protein) Is Required for Actin Cone Movement during Spermatid Individualization inInternational journal of molecular sciences · 2022Article
- Diverse functions of myosin VI in spermiogenesis.Histochemistry and cell biology · 2021Review
- Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis†.Biology of reproduction · 2020Article
- Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis†‡.Biology of reproduction · 2020Article
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Article
- Myosin 1b is an actin depolymerase.Nature communications · 2019Article
- Combover interacts with the axonemal component Rsp3 and is required forDevelopment (Cambridge, England) · 2019Article
- Article
- Distinct roles of two myosins in C. elegans spermatid differentiation.PLoS biology · 2019Article
- Drosophila Pif1A is essential for spermatogenesis and is the homolog of human CCDC157, a gene associated with idiopathic NOA.Cell death & disease · 2019Article
- Myosin VI-Dependent Actin Cages Encapsulate Parkin-Positive Damaged Mitochondria.Developmental cell · 2018Article
- Expression and localization of myosin VI in developing mouse spermatids.Histochemistry and cell biology · 2017Article
- Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios.Development (Cambridge, England) · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Here, we demonstrate a new function of myosin VI using observations of Drosophila spermatid individualization in vivo. We find that myosin VI stabilizes a branched actin network in actin structures (cones) that mediate the separation of the syncytial spermatids. In a myosin VI mutant, the cones do not accumulate F-actin during cone movement, whereas overexpression of myosin VI leads to bigger cones with more F-actin. Myosin subfragment 1-fragment decoration demonstrated that the actin cone is made up of two regions: a dense meshwork at the front and parallel bundles at the rear. The majority of the actin filaments were oriented with their pointed ends facing in the direction of cone movement. Our data also demonstrate that myosin VI binds to the cone front using its motor domain. Fluorescence recovery after photobleach experiments using green fluorescent protein-myosin VI revealed that myosin VI remains bound to F-actin for minutes, suggesting its role is tethering, rather than transporting cargo. We hypothesize that myosin VI protects the actin cone structure either by cross-linking actin filaments or anchoring regulatory molecules at the cone front. These observations uncover a novel mechanism mediated by myosin VI for stabilizing long-lived actin structures in cells.
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Registered trials
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