ArticleWorld journal of gastroenterology2006
Reduced expression of beta-catenin inhibitor Chibby in colon carcinoma cell lines.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Multi-omics evidence reveals a causal role of endoplasmic reticulum stress in cancer development.PeerJ · 2026Article
- Identifying biomarkers of endoplasmic reticulum stress and analyzing immune cell infiltration in chronic obstructive pulmonary disease using machine learning.Frontiers in medicine · 2024Article
- A novel Lnc408 maintains breast cancer stem cell stemness by recruiting SP3 to suppress CBY1 transcription and increasing nuclear β-catenin levels.Cell death & disease · 2021Article
- Chibby suppresses aerobic glycolysis and proliferation of nasopharyngeal carcinoma via the Wnt/β-catenin-Lin28/let7-PDK1 cascade.Journal of experimental & clinical cancer research : CR · 2018Article
- Anti-oncogenic activity of Chibby in the development of human nasopharyngeal carcinoma.Oncology letters · 2018Article
- Downregulation of nuclear and cytoplasmic Chibby is associated with advanced cervical cancer.Oncology letters · 2017Article
- Review
- Chibby1 knockdown promotes mesenchymal-to-epithelial transition-like changes.Cell cycle (Georgetown, Tex.) · 2017Article
- BCR-ABL1-associated reduction of beta catenin antagonist Chibby1 in chronic myeloid leukemia.PloS one · 2013Article
- Chibby suppresses growth of human SW480 colon adenocarcinoma cells through inhibition of β-catenin signaling.Journal of molecular signaling · 2012Article
- Structural characterization of partially disordered human Chibby: insights into its function in the Wnt-signaling pathway.Biochemistry · 2011Article
- Fine-tuning of nuclear-catenin by Chibby and 14-3-3.Cell cycle (Georgetown, Tex.) · 2009Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo analyse the Chibby expression and its function in colon carcinoma cell lines and colorectal carcinoma (CRC).
methodsChibby expression levels were investigated by quantitative RT-PCR in a panel of seven different colon carcinoma cell lines. By sequencing, we analysed mutational status of Chibby. To test whether Chibby exhibited effects on beta-catenin signalling in colon carcinoma cells, we transfected SW480 cells with Chibby expression plasmid and, subsequently, analysed activity of beta-catenin and tested for alterations in cellular phenotype. In addition, we examined Chibby mRNA levels in samples of colorectal carcinomas and adjacent normal tissues by using quantitative RT-PCR and hybridised gene chips with samples from CRC and normal tissues.
resultsChibby mRNA expression was strongly down-regulated in colon carcinoma cell lines in comparison to normal colon epithelial cells and no mutation in any of the examined colon carcinoma cell lines was found. Further, we could show that Chibby inhibited beta-catenin activity in TOPflash assays when over-expressed in SW480 cells. Proliferation and invasion assays with Chibby transfected SW480 cells did not reveal profound differences compared to control cells. In contrast to these in vitro data, quantitative RT-PCR analyses of Chibby mRNA levels in CRC tumor samples did not show significant differences to specimens in adjacent non-cancerous tissue. Consistent with these findings, gene chips analysing tissue samples of tumors and corresponding normal tissue did not show altered Chibby expression.
conclusionAltered Chibby expression might be observed in vitro in different colon carcinoma cell lines. However, this finding could not be confirmed in vitro in CRC tumors, indicating that Chibby is not likely to promote CRC tumor development or progression. As Chibby is an important inhibitor of beta-catenin signalling, our data implicate that the usability of colon carcinoma cell lines for in vitro studies analysing the Wnt/beta-catenin pathway in colorectal carcinoma needs extensive verification.
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