ArticleClinical and molecular allergy : CMA2006
House dust mite major allergens Der p 1 and Der p 5 activate human airway-derived epithelial cells by protease-dependent and protease-independent mechanisms.
Article in Clinical and molecular allergy : CMA, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 167 citations in OpenAlex.
- Mechanistic modelling of allergen-induced airways disease in early life.Scientific reports · 2025Article
- Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research.Respiratory research · 2024Article
- ATP functions as a primary alarmin in allergen-induced type 2 immunity.American journal of physiology. Cell physiology · 2023Review
- Circadian regulation of pulmonary disease: the importance of timing.Clinical science (London, England : 1979) · 2023Review
- Beyond allergic progression: From molecules to microbes as barrier modulators in the gut-lung axis functionality.Frontiers in allergy · 2023Review
- Acute and chronic lung inflammation drives changes in epithelial glycans.Frontiers in immunology · 2023Article
- Toll-like receptor 4 and Syk kinase shape dendritic cell-induced immune activation to major house dust mite allergens.Frontiers in medicine · 2023Article
- Allergen Content and Protease Activity in Milk Feeds from Mothers of Preterm Infants.Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine · 2022Observational
- Molecular Characterization of Kunitz-Type Protease Inhibitors from Blister Beetles (Coleoptera, Meloidae).Biomolecules · 2022Article
- Mechanisms and pathogenesis of chronic rhinosinusitis.The Journal of allergy and clinical immunology · 2022Review
- Protease-activated receptor-2: Role in asthma pathogenesis and utility as a biomarker of disease severity.Frontiers in medicine · 2022Review
- Biological Response-Enhancing Activity with Antigens in A549 Cells Exposed to Representative Polycyclic Aromatic Hydrocarbons.ACS omega · 2021Article
- IL-4Rα blockade reduces influenza-associated morbidity in a murine model of allergic asthma.Respiratory research · 2021Article
- Der p 1 Disrupts the Epithelial Barrier and Induces IL-6 Production in Patients With House Dust Mite Allergic Rhinitis.Frontiers in allergy · 2021Article
- Interleukin-28B dampens protease-induced lung inflammation via IL-25 and TSLP inhibition in epithelial cells.Scientific reports · 2020Article
- Degradation of bacterial permeability family member A1 (BPIFA1) by house dust mite (HDM) cysteine protease Der p 1 abrogates immune modulator function.International journal of biological macromolecules · 2020Article
- Review
- Review
- Alternaria induces airway epithelial cytokine expression independent of protease-activated receptor.Respirology (Carlton, Vic.) · 2020Article
- The allergenic activity and clinical impact of individual IgE-antibody binding molecules from indoor allergen sources.The World Allergy Organization journal · 2020Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
House dust mite allergens (HDM) cause bronchoconstriction in asthma patients and induce an inflammatory response in the lungs due to the release of cytokines, chemokines and additional mediators. The mechanism how HDM components achieve this is largely unknown. The objective of this study was to assess whether HDM components of Dermatophagoides pteronissinus with protease activity (Der p 1) and unknown enzymatic activity (Der p 2, Der p 5) induce biological responses in a human airway-derived epithelial cell line (A549), and if so, to elucidate the underlying mechanism(s) of action. A549 cells were incubated with HDM extract, Der p 1, recombinant Der p 2 and recombinant Der p 5. Cell desquamation was assessed by microscopy. The proinflammatory cytokines, IL-6 and IL-8, were measured by ELISA. Intracellular Ca2+ levels were assessed in A549 cells and in mouse fibroblasts expressing the human protease activated receptor (PAR)1, PAR2 or PAR4. HDM extract, Der p 1 and Der p 5 dose-dependently increased the production of IL-6 and IL-8. Added simultaneously, Der p 1 and Der p 5 further increased the production of IL-6 and IL-8. The action of Der p 1 was blocked by cysteine-protease inhibitors, while that of Der p 5 couldn't be blocked by either serine- or cysteine protease inhibitors. Der p 5 only induced cell shrinking, whereas HDM extract and Der p1 also induced cell desquamation. Der p 2 had no effect on A549 cells. Der p 1's protease activity causes desquamation and induced the release of IL6 and IL-8 by a mechanism independent of Ca2+ mobilisation and PAR activation. Der p 5 exerts a protease-independent activation of A549 that involves Ca2+ mobilisation and also leads to the production of these cytokines. Together, our data indicate that allergens present in HDM extracts can trigger protease-dependent and protease-independent signalling pathways in A549 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.