ArticleProceedings of the National Academy of Sciences of the United States of America2006
Suppression of prion protein in livestock by RNA interference.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 138 citations in OpenAlex.
- Prions and protein aggregates as pathogens, self-propagating structures, biomarkers, and therapeutic targets.Microbiology and molecular biology reviews : MMBR · 2025Review
- Prion diseases: Lessons from historical outbreaks and potential emerging ones.Protein science : a publication of the Protein Society · 2025Review
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- New implications for prion diseases therapy and prophylaxis.Frontiers in molecular neuroscience · 2024Review
- Genetic resilience in chickens against bacterial, viral and protozoal pathogens.Frontiers in veterinary science · 2022Review
- Elucidating Critical Proteinopathic Mechanisms and Potential Drug Targets in Neurodegeneration.Cellular and molecular neurobiology · 2020Review
- Harnessing genomic information for livestock improvement.Nature reviews. Genetics · 2019Review
- Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era.Frontiers in genetics · 2019Review
- Generation of genetically-engineered animals using engineered endonucleases.Archives of pharmacal research · 2018Review
- Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line.Transgenic research · 2017Article
- Genetically engineered livestock for agriculture: a generation after the first transgenic animal research conference.Transgenic research · 2016Review
- Small Non-coding RNAs Associated with Viral Infectious Diseases of Veterinary Importance: Potential Clinical Applications.Frontiers in veterinary science · 2016Review
- Role of calpastatin in the regulation of mRNA expression of calpain, caspase, and heat shock protein systems in bovine muscle satellite cells.In vitro cellular & developmental biology. Animal · 2015Article
- Significant role of μ-calpain (CANP1) in proliferation/survival of bovine skeletal muscle satellite cells.In vitro cellular & developmental biology. Animal · 2013Article
- Transgenically mediated shRNAs targeting conserved regions of foot-and-mouth disease virus provide heritable resistance in porcine cell lines and suckling mice.Veterinary research · 2013Article
- Progress and prospects for genetic modification of nonhuman primate models in biomedical research.ILAR journal · 2013Review
- Article
- Transgenesis applied to goat: current applications and ongoing research.Transgenic research · 2012Review
- Targeted microRNA expression in dairy cattle directs production of β-lactoglobulin-free, high-casein milk.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Inhibition of porcine reproductive and respiratory syndrome virus replication by RNA interference in MARC-145 cells.Molecular biology reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Given the difficulty of applying gene knockout technology to species other than mice, we decided to explore the utility of RNA interference (RNAi) in silencing the expression of genes in livestock. Short hairpin RNAs (shRNAs) were designed and screened for their ability to suppress the expression of caprine and bovine prion protein (PrP). Lentiviral vectors were used to deliver a transgene expressing GFP and an shRNA targeting PrP into goat fibroblasts. These cells were then used for nuclear transplantation to produce a cloned goat fetus, which was surgically recovered at 81 days of gestation and compared with an age-matched control derived by natural mating. All tissues examined in the cloned fetus expressed GFP, and PCR analysis confirmed the presence of the transgene encoding the PrP shRNA. Most relevant, Western blot analysis performed on brain tissues comparing the transgenic fetus with control demonstrated a significant (>90%) decrease in PrP expression levels. To confirm that similar methodologies could be applied to the bovine, recombinant virus was injected into the perivitelline space of bovine ova. After in vitro fertilization and culture, 76% of the blastocysts exhibited GFP expression, indicative that they expressed shRNAs targeting PrP. Our results provide strong evidence that the approach described here will be useful in producing transgenic livestock conferring potential disease resistance and provide an effective strategy for suppressing gene expression in a variety of large-animal models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.