ArticleThe Journal of cell biology2006
Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- Emerging connections between GPI-anchored proteins and their extracellular carriers in colorectal cancer.Extracellular vesicles and circulating nucleic acids · 2023Article
- Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells.Current biology : CB · 2022Article
- Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.Frontiers in cell and developmental biology · 2022Article
- Calcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
- Endoplasmic Reticulum Export of GPI-Anchored Proteins.International journal of molecular sciences · 2019Review
- Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Trafficking of glycosylphosphatidylinositol anchored proteins from the endoplasmic reticulum to the cell surface.Journal of lipid research · 2016Review
- Dual pulse-chase microscopy reveals early divergence in the biosynthetic trafficking of the Na,K-ATPase and E-cadherin.Molecular biology of the cell · 2015Article
- C. elegans as a model for membrane traffic.WormBook : the online review of C. elegans biology · 2014Review
- Mass spectrometry-based targeted proteomics as a tool to elucidate the expression and function of intestinal drug transporters.The AAPS journal · 2013Review
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- 3D single molecule tracking with multifocal plane microscopy reveals rapid intercellular transferrin transport at epithelial cell barriers.Biophysical journal · 2012Article
- Eisosome proteins assemble into a membrane scaffold.The Journal of cell biology · 2011Article
- 3D single molecule tracking of quantum-dot labeled antibody molecules using multifocal plane microscopy.Proceedings of SPIE--the International Society for Optical Engineering · 2010Article
- Clathrin and AP1B: key roles in basolateral trafficking through trans-endosomal routes.FEBS letters · 2009Review
- Transport of mannose-6-phosphate receptors from the trans-Golgi network to endosomes requires Rab31.Experimental cell research · 2009Article
- Taking the scenic route: biosynthetic traffic to the plasma membrane in polarized epithelial cells.Traffic (Copenhagen, Denmark) · 2009Review
- Membrane proteins follow multiple pathways to the basolateral cell surface in polarized epithelial cells.The Journal of cell biology · 2009Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Although epithelial cells are known to exhibit a polarized distribution of membrane components, the pathways responsible for delivering membrane proteins to their appropriate domains remain unclear. Using an optimized approach to three-dimensional live cell imaging, we have visualized the transport of newly synthesized apical and basolateral membrane proteins in fully polarized filter-grown Madin-Darby canine kidney cells. We performed a detailed quantitative kinetic analysis of trans-Golgi network (TGN) exit, passage through transport intermediates, and arrival at the plasma membrane using cyan/yellow fluorescent protein-tagged glycosylphosphatidylinositol-anchored protein and vesicular stomatitis virus glycoprotein as apical and basolateral reporters, respectively. For both pathways, exit from the TGN was rate limiting. Furthermore, apical and basolateral proteins were targeted directly to their respective membranes, resolving current confusion as to whether sorting occurs on the secretory pathway or only after endocytosis. However, a transcytotic protein did reach the apical surface after a prior appearance basolaterally. Finally, newly synthesized proteins appeared to be delivered to the entire lateral or apical surface, suggesting-contrary to expectations-that there is not a restricted site for vesicle docking or fusion adjacent to the junctional complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.