ArticleMatrix biology : journal of the International Society for Matrix Biology2006
The N-terminal 70-kDa fragment of fibronectin binds to cell surface fibronectin assembly sites in the absence of intact fibronectin.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 60 citations in OpenAlex.
- SOCS domain targets ECM assembly in lung fibroblasts and experimental lung fibrosis.Scientific reports · 2024Article
- ADAMTS18-fibronectin interaction regulates the morphology of liver sinusoidal endothelial cells.iScience · 2024Article
- [Science advances · 2024Article
- SOCS domain targets ECM assembly in lung fibroblasts and experimental lung fibrosis.bioRxiv : the preprint server for biology · 2024Article
- Fibronectin in hyperglycaemia and its potential use in the treatment of diabetic foot ulcers: A review.International wound journal · 2023Review
- A new mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy.Journal of cell science · 2022Article
- The interactions between integrin αInternational journal of biological sciences · 2022Article
- Article
- Fibronectin assembly regulates lumen formation in breast acini.Journal of cellular biochemistry · 2021Article
- The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.Scientific reports · 2020Article
- Article
- TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanism.Molecular biology of the cell · 2017Article
- Myofibroblasts exhibit enhanced fibronectin assembly that is intrinsic to their contractile phenotype.The Journal of biological chemistry · 2015Article
- Bivalent ligation of the collagen-binding modules of fibronectin by SFS, a non-anchored bacterial protein of Streptococcus equi.The Journal of biological chemistry · 2015Article
- Article
- Factor XIII and adipocyte biology.Blood · 2014Article
- Borrelia burgdorferi protein BBK32 binds to soluble fibronectin via the N-terminal 70-kDa region, causing fibronectin to undergo conformational extension.The Journal of biological chemistry · 2014Article
- Nonselective assembly of fibrillin 1 and fibrillin 2 in the rodent ocular zonule and in cultured cells: implications for Marfan syndrome.Investigative ophthalmology & visual science · 2013Article
- Complex contributions of fibronectin to initiation and maturation of microfibrils.The Biochemical journal · 2013Article
- Characterization of molecules binding to the 70K N-terminal region of fibronectin by IFAST purification coupled with mass spectrometry.Journal of proteome research · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Binding of the N-terminal 70-kDa (70K) fragment of fibronectin to fibroblasts blocks assembly of intact fibronectin and is an accurate indicator of the ability of various agents to enhance or inhibit fibronectin assembly. Such binding is widely thought to be to already assembled fibronectin. We evaluated this hypothesis with fibronectin-null mouse fibroblasts plated on laminin-1 in the absence of intact fibronectin. As a proteolytic fragment or recombinant protein, 70K bound fibronectin-null cells specifically in linear arrays that extended outwards from the periphery of spread cells. At early time points, these arrays were similar to those formed by intact fibronectin. 70K arrays formed within 5 min following ligand addition at concentrations as low as 5 nM, indicating rapid and high affinity binding. Bound 70K was extractable with Triton X-100 or deoxycholate but became insoluble when cross-linked with a membrane-impermeable agent into large SDS-stable complexes. Intact fibronectin, in contrast, became progressively non-extractable in the absence of cross-linking. The detergent-resistant arrays of cross-linked 70K localized to tips of cellular extensions and partially overlapped with alpha6 and beta1 integrin subunits at the base of the extensions. alpha5 did not localize with 70K arrays, but became progressively co-localized with assemblies of intact fibronectin over time. These results support a model in which the 70-kDa region of fibronectin binds to linearly arrayed cell surface molecules of adherent cells to initiate assembly, display of the arrays is controlled by the integrin that mediates adhesion, and fibronectin-binding integrins promote fibronectin-fibronectin interactions during progression of assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.