ArticleLangenbeck's archives of surgery2006
Isolation and transduction of monocytes: promising vehicles for therapeutic arteriogenesis.
Article in Langenbeck's archives of surgery, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- Negative Magnetic Sorting Preserves the Functionality of Ex Vivo Cultivated Non-Adherent Human Monocytes.Biology · 2022Article
- Mimicking of Blood Flow Results in a Distinct Functional Phenotype in Human Non-Adherent Classical Monocytes.Biology · 2021Article
- Clinical improvement and enhanced collateral vessel growth after xenogenic monocyte transplantation.American journal of translational research · 2019Article
- Isolation and intravenous injection of murine bone marrow derived monocytes.Journal of visualized experiments : JoVE · 2014Article
- Novel ex vivo culture method for human monocytes uses shear flow to prevent total loss of transendothelial diapedesis function.Journal of leukocyte biology · 2014Article
- The effect of cyclic mechanical strain on activation of dendritic cells cultured on adhesive substrates.Biomaterials · 2013Article
- The generation of NGF-secreting primary rat monocytes: a comparison of different transfer methods.Journal of immunological methods · 2013Article
- Transplantation of bone marrow derived monocytes: a novel approach for augmentation of arteriogenesis in a murine model of femoral artery ligation.American journal of translational research · 2013Article
- Circumventing antivector immunity by using adenovirus-infected blood cells for repeated application of adenovirus-vectored vaccines: proof of concept in rhesus macaques.Journal of virology · 2012Article
- Monocyte transplantation for neural and cardiovascular ischemia repair.Journal of cellular and molecular medicine · 2010Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsAugmentation of collateral vessel growth (arteriogenesis) is of particular clinical interest for the treatment of vascular occlusive disease. Monocytes play a key role for arteriogenesis. They localize to areas of collateral development and create a highly arteriogenic environment. "Homing" of ex vivo genetically engineered monocytes could therapeutically be exploited for augmentation of arteriogenesis. However, isolation and ex vivo transduction of monocytes is problematic.
methodsIn this study, we established a valid method of monocyte isolation from peripheral blood and evaluated different in vitro transduction methods.
resultsOur results revealed that liposomes and electroporation were unsuccessful for monocyte transduction. However, high-efficiency gene transfer (almost 95%) was achieved by adenoviral infection. Subsequent homing of virally transduced monocytes to sites of arteriogenesis could be demonstrated.
conclusionOur study may offer a new method for the augmentation of arteriogenesis, all of which makes the ultimate goal of applying this strategy to humans for therapy of vascular disease eminently attractive.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.