ArticleBritish journal of pharmacology2006
Involvement of Rho kinase and protein kinase C in carbachol-induced calcium sensitization in beta-escin skinned rat and guinea-pig bladders.
Article in British journal of pharmacology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 45 citations in OpenAlex.
- The role of rhoA/rho-kinase and PKC in the inhibitory effect of L-cysteine/HNaunyn-Schmiedeberg's archives of pharmacology · 2023Article
- Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology.BMC urology · 2015Review
- Aging changes agonist induced contractile responses in permeabilized rat bladder.Age (Dordrecht, Netherlands) · 2015Article
- Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle.The Journal of physiology · 2012Article
- Endogenous cardiac troponin T modulates Ca(2+)-mediated smooth muscle contraction.Scientific reports · 2012Article
- The Relaxing Effect of α-Defensin 1 on the Adrenergic Responses of Rat Bladder.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2011Article
- Calcium sensitization induced by sodium fluoride in permeabilized rat mesenteric arteries.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2010Article
- Alteration of the PKC-mediated signaling pathway for smooth muscle contraction in obstruction-induced hypertrophy of the urinary bladder.Laboratory investigation; a journal of technical methods and pathology · 2009Article
- Synergistic effects of cyclic strain and Th1-like cytokines on tenascin-C production by rheumatic aortic valve interstitial cells.Clinical and experimental immunology · 2009Article
- The effects of reactive oxygen species on calcium- and carbachol-induced contractile responses in beta-escin permeabilized rat bladder.Naunyn-Schmiedeberg's archives of pharmacology · 2008Article
- Signal transduction underlying the control of urinary bladder smooth muscle tone by muscarinic receptors and beta-adrenoceptors.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
- Evolving mechanisms of action of alverine citrate on phasic smooth muscles.British journal of pharmacology · 2007Article
- Comparative pharmacological analysis of Rho-kinase inhibitors and identification of molecular components of Ca2+ sensitization in the rat lower urinary tract.Biochemical pharmacology · 2007Article
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2 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
1. The signal transduction pathways involved in carbachol (CCh)-induced calcium sensitization in beta-escin permeabilized rat and guinea-pig bladder smooth muscles were investigated and the results were compared with guinea-pig taenia caecum. 2. Calcium contractions elicited cumulatively (pCa 7.5-5) in the presence of calmodulin were significantly increased in all three tissues when CCh (50 microM) was added to the medium. 3. Under constant [Ca2+]i conditions (pCa 6), calmodulin (1 microM) and then GTP (100 microM) initiated significant contractions. CCh (50 microM) added to the bath caused a further contraction in all three tissues - calcium sensitization. This sensitization was significantly inhibited by atropine (50 microM). 4. The incubation of the tissues with the IP3-receptor blocker 2-APB (30 microM) reduced the subsequent development of calcium sensitization by CCh in rat bladder but did not affect it in guinea-pig bladder and taenia ceacum. 5. The Rho kinase (ROK) inhibitor Y-27632 (5 microM) added in the presence of CCh reversed the calcium sensitization in rat bladder, whereas a transient contraction followed by a relaxation to a level not significantly different from the CCh contraction was seen in both guinea-pig bladder and taenia caecum. Y-27632 (1 microM) continuously present significantly inhibited the CCh-induced Ca2+ sensitization in rat bladder but not in guinea-pig bladder or taenia caecum. 6. In the presence of cyclopiazonic acid (CPA) (1 microM) and calmodulin (1 microM), Y-27632 (5 microM) did not change the calcium response curve (3 x 10(-7)-10(-5) M) in rat bladder but increased the contractile responses significantly in both guinea-pig bladder and taenia caecum. 7. The protein kinase C (PKC) inhibitor GF 109203X (5 microM) added in the presence of CCh inhibited the calcium sensitization induced by this muscarinic agonist in all three tissues in different ratios. 8. In conclusion, muscarinic receptor activation induces calcium sensitization in rat and guinea-pig detrusor smooth muscles but there are differences in their pathways.
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