ArticleBiophysical journal2006
Cytochrome C interaction with cardiolipin/phosphatidylcholine model membranes: effect of cardiolipin protonation.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Order-to-Disorder and Disorder-to-Order Transitions of Proteins upon Binding to Phospholipid Membranes: Common Ground and Dissimilarities.Biomolecules · 2025Review
- The Increase in the Peroxidase Activity of the CytochromeInternational journal of molecular sciences · 2024Article
- Mitochondrial membrane model: Lipids, elastic properties, and the changing curvature of cardiolipin.Biophysical journal · 2023Article
- Composition of the CytochromeMolecules (Basel, Switzerland) · 2023Article
- Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs.Membranes · 2023Article
- Review
- The Puzzling Problem of Cardiolipin Membrane-Cytochrome c Interactions: A Combined Infrared and Fluorescence Study.International journal of molecular sciences · 2021Article
- Wheel and Deal in the Mitochondrial Inner Membranes: The Tale of CytochromeOxidative medicine and cellular longevity · 2020Review
- pH-Induced Switch between Different Modes of CytochromeACS omega · 2019Article
- Cardiolipin Structure and Oxidation Are Affected by CaFrontiers in chemistry · 2019Article
- Relating the multi-functionality of cytochrome c to membrane binding and structural conversion.Biophysical reviews · 2018Review
- Magic angle spinningBiochimica et biophysica acta. Biomembranes · 2017Article
- Nanodisc Films for Membrane Protein Studies by Neutron Reflection: Effect of the Protein Scaffold Choice.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Ionization constants pKa of cardiolipin.PloS one · 2013Article
- Lytic and non-lytic permeabilization of cardiolipin-containing lipid bilayers induced by cytochrome C.PloS one · 2013Article
- Versatility of non-native forms of human cytochrome c: pH and micellar concentration dependence.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2013Article
- Cytochrome c-lipid interactions: new insights from resonance energy transfer.Biophysical journal · 2010Article
- Cytochrome c/cardiolipin relations in mitochondria: a kiss of death.Free radical biology & medicine · 2009Review
- A comparison of the membrane binding properties of C1B domains of PKCgamma, PKCdelta, and PKCepsilon.Biophysical journal · 2009Article
- Assigning membrane binding geometry of cytochrome C by polarized light spectroscopy.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resonance energy transfer between anthrylvinyl-labeled phosphatidylcholine as a donor and heme moiety of cytochrome c (cyt c) as an acceptor has been employed to explore the protein binding to model membranes, composed of phosphatidylcholine and cardiolipin (CL). The existence of two types of protein-lipid complexes has been hypothesized where either deprotonated or partially protonated CL molecules are responsible for cyt c attachment to bilayer surface. To quantitatively describe cyt c membrane binding, the adsorption model based on scaled particle and double layer theories has been employed, with potential-dependent association constants being treated as a function of acidic phospholipid mole fraction, degree of CL protonation, ionic strength, and surface coverage. Multiple arrays of resonance energy transfer data obtained under conditions of varying pH, ionic strength, CL content, and protein/lipid molar ratio have been analyzed in terms of the model of energy transfer in two-dimensional systems combined with the adsorption model allowing for area exclusion and electrostatic effects. The set of recovered model parameters included effective protein charge, intrinsic association constants, and heme distance from the bilayer midplane for both types of protein-lipid complexes. Upon increasing CL mole fraction from 10 to 20 mol % (the value close to that characteristic of the inner mitochondrial membrane), the binding equilibrium dramatically shifted toward cyt c association with partially protonated CL species. The estimates of heme distance from bilayer center suggest shallow bilayer location of cyt c at physiological pH, whereas at pH below 6.0, the protein tends to insert into membrane core.
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