ArticleProceedings of the National Academy of Sciences of the United States of America1991
Identification of 1,4-dihydropyridine binding regions within the alpha 1 subunit of skeletal muscle Ca2+ channels by photoaffinity labeling with diazipine.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 145 citations in OpenAlex.
- The molecular transition that confers voltage dependence to muscle contraction.Nature communications · 2025Article
- Voltage gated sodium and calcium channels: Discovery, structure, function, and Pharmacology.Channels (Austin, Tex.) · 2023Review
- Structural model for phenylalkylamine binding to L-type calcium channels.The Journal of biological chemistry · 2009Article
- L-type voltage-dependent calcium channel antagonists impair perirhinal long-term recognition memory and plasticity processes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Functional architecture of the inner pore of a voltage-gated Ca2+ channel.The Journal of general physiology · 2005Article
- Efficient identification of photolabelled amino acid residues by combining immunoaffinity purification with MS: revealing the semotiadil-binding site and its relevance to binding sites for myristates in domain III of human serum albumin.The Biochemical journal · 2002Article
- Modulation of the synaptic Ca2+ current in salamander photoreceptors by polyunsaturated fatty acids and retinoids.The Journal of physiology · 2000Article
- Molecular basis of drug interaction with L-type Ca2+ channels.Journal of bioenergetics and biomembranes · 1998Review
- Fatty acids suppress voltage-gated Na+ currents in HEK293t cells transfected with the alpha-subunit of the human cardiac Na+ channel.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Construction of a high-affinity receptor site for dihydropyridine agonists and antagonists by single amino acid substitutions in a non-L-type Ca2+ channel.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Voltage-dependent binding and calcium channel current inhibition by an anti-alpha 1D subunit antibody in rat dorsal root ganglion neurones and guinea-pig myocytes.The Journal of physiology · 1997Article
- Suppression of voltage-gated L-type Ca2+ currents by polyunsaturated fatty acids in adult and neonatal rat ventricular myocytes.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Dihydropyridine action on voltage-dependent potassium channels expressed in Xenopus oocytes.The Journal of general physiology · 1997Article
- A Drosophila calcium channel alpha1 subunit gene maps to a genetic locus associated with behavioral and visual defects.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Effects of calciseptine on unitary barium channel currents in guinea-pig portal vein.Pflugers Archiv : European journal of physiology · 1996Article
- The IVS6 segment of the L-type calcium channel is critical for the action of dihydropyridines and phenylalkylamines.The EMBO journal · 1996Article
- Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Calcium current reactivation after flash photolysis of nifedipine in skeletal muscle fibres of the frog.The Journal of physiology · 1995Article
- Synthesis of nitrodiazirinyl derivatives of neurotoxin II from Naja naja oxiana and their interaction with the Torpedo californica nicotinic acetylcholine receptor.Journal of protein chemistry · 1995Article
- A mutation in segment IVS6 disrupts fast inactivation of sodium channels.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To identify regions that are involved in the formation of the dihydropyridine receptor site of skeletal muscle L-type Ca2+ channels, the alpha 1 subunit of the channel complex was specifically labeled with the 1,4-dihydropyridine-receptor-selective photoaffinity probe [3H]diazipine. Photoaffinity-labeled regions were identified by probing labeled proteolytic fragments with several anti-peptide antibodies recognizing different segments of the alpha 1 sequence. Forty to 50% of the alpha 1-associated [3H]diazipine label was contained in the tryptic fragment between Arg-988 and Ala-1023 derived from the loop between segments S5 and S6 in domain III. This region corresponds to a portion of the channel that is believed to contribute to formation of the transmembrane pore. Twenty to 30% of the labeling occurred in a V8 protease fragment between Glu-1349 and Trp-1391. This fragment contains transmembrane segment S6 of domain IV and has previously been shown to form part of the drug receptor for phenylalkylamine Ca2+ antagonists. Our data suggest that the dihydropyridine receptor is formed by close apposition of two discontinuous regions of the alpha 1 subunit sequence in domains III and IV. In light of previous work localizing this receptor site to the extracellular surface of the lipid bilayer, it is proposed that amino acid residues at the extracellular surface in the loop connecting segments IIIS5 and IIIS6 and at the extracellular end of segment IVS6 contribute to formation of the dihydropyridine receptor site.
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