ArticleRNA (New York, N.Y.)2006
Identification of nucleotides in E. coli 16S rRNA essential for ribosome subunit association.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Molecular mechanism of IF1- and IF2-driven translation initiation in bacteria.bioRxiv : the preprint server for biology · 2026Article
- Three-dimensional structure-guided evolution of a ribosome with tethered subunits.Nature chemical biology · 2022Article
- Hibernation factors directly block ribonucleases from entering the ribosome in response to starvation.Nucleic acids research · 2021Article
- Exploring allosteric communication in multiple states of the bacterial ribosome using residue network analysis.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
- Intersubunit Bridges of the Bacterial Ribosome.Journal of molecular biology · 2016Review
- Dynamic contact network between ribosomal subunits enables rapid large-scale rotation during spontaneous translocation.Nucleic acids research · 2015Article
- The emerging role of rectified thermal fluctuations in initiator aa-tRNA- and start codon selection during translation initiation.Biochimie · 2015Review
- The effects of disruptions in ribosomal active sites and in intersubunit contacts on ribosomal degradation in Escherichia coli.Scientific reports · 2015Article
- Real-time evidence for EF-G-induced dynamics of helix 44 in 16S rRNA.Journal of molecular biology · 2012Article
- Article
- Mutations in the intersubunit bridge regions of 16S rRNA affect decoding and subunit-subunit interactions on the 70S ribosome.Nucleic acids research · 2011Article
- Differential assembly of 16S rRNA domains during 30S subunit formation.RNA (New York, N.Y.) · 2010Article
- Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.Applied and environmental microbiology · 2008Article
- Revealing unique properties of the ribosome using a network based analysis.Nucleic acids research · 2008Article
- Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin.Antimicrobial agents and chemotherapy · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ribosome consists of two unequal subunits, which associate via numerous intersubunit contacts. Medium-resolution structural studies have led to grouping of the intersubunit contacts into 12 directly visualizable intersubunit bridges. Most of the intersubunit interactions involve RNA. We have used an RNA modification interference approach to determine Escherichia coli 16S rRNA positions that are essential for the association of functionally active 70S ribosomes. Modification of the N1 position of A702, A1418, and A1483 with DMS, and of the N3 position of U793, U1414, and U1495 with CMCT in 30S subunits strongly interferes with 70S ribosome formation. Five of these positions localize into previously recognized intersubunit bridges, namely, B2a (U1495), B2b (U793), B3 (A1483), B5 (A1418), and B7a (A702). The remaining position displaying interference, U1414, forms a base pair with G1486, which is a part of bridge B3. We contend that these five intersubunit bridges are essential for reassociation of the 70S ribosome, thus forming the functional core of the intersubunit contacts.
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