ArticleNucleic acids research2006
Protein assembly and DNA looping by the FokI restriction endonuclease.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction-modification enzyme.Nucleic acids research · 2025Article
- A sequential one-pot approach for rapid and convenient characterization of putative restriction-modification systems.mSystems · 2023Article
- Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI.Nucleic acids research · 2023Article
- Review
- Review
- A single active site in the mariner transposase cleaves DNA strands of opposite polarity.Nucleic acids research · 2017Article
- Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.Nucleic acids research · 2017Article
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- Organization of the BcgI restriction-modification protein for the cleavage of eight phosphodiester bonds in DNA.Nucleic acids research · 2013Article
- TALE-PvuII fusion proteins--novel tools for gene targeting.PloS one · 2013Article
- DNA looping by FokI: the impact of twisting and bending rigidity on protein-induced looping dynamics.Nucleic acids research · 2012Article
- DNA looping by FokI: the impact of synapse geometry on loop topology at varied site orientations.Nucleic acids research · 2012Article
- Monomeric site-specific nucleases for genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A novel zinc-finger nuclease platform with a sequence-specific cleavage module.Nucleic acids research · 2012Article
- Illuminating the reaction pathway of the FokI restriction endonuclease by fluorescence resonance energy transfer.Nucleic acids research · 2012Article
- Protein sliding and DNA denaturation are essential for DNA organization by human mitochondrial transcription factor A.Nature communications · 2012Article
- Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation.Nucleic acids research · 2011Article
- Concerted action at eight phosphodiester bonds by the BcgI restriction endonuclease.Nucleic acids research · 2011Article
- Adding fingers to an engineered zinc finger nuclease can reduce activity.Biochemistry · 2011Article
- Creating designed zinc-finger nucleases with minimal cytotoxicity.Journal of molecular biology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The FokI restriction endonuclease recognizes an asymmetric DNA sequence and cuts both strands at fixed positions upstream of the site. The sequence is contacted by a single monomer of the protein, but the monomer has only one catalytic centre and forms a dimer to cut both strands. FokI is also known to cleave DNA with two copies of its site more rapidly than DNA with one copy. To discover how FokI acts at a single site and how it acts at two sites, its reactions were examined on a series of plasmids with either one recognition site or with two sites separated by varied distances, sometimes in the presence of a DNA-binding defective mutant of FokI. These experiments showed that, to cleave DNA with one site, the monomer bound to that site associates via a weak protein-protein interaction with a second monomer that remains detached from the recognition sequence. Nevertheless, the second monomer catalyses phosphodiester bond hydrolysis at the same rate as the DNA-bound monomer. On DNA with two sites, two monomers of FokI interact strongly, as a result of being tethered to the same molecule of DNA, and sequester the intervening DNA in a loop.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.