ArticleProteome science2006
Optimising the use of TRIzol-extracted proteins in surface enhanced laser desorption/ ionization (SELDI) analysis.
Article in Proteome science, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Specimen Inactivation Methods for Proteomics─Comparisons of Irradiation, Chemical, and Heat Treatments on Downstream Serum Analyses.Journal of proteome research · 2025Article
- High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction.BioTechniques · 2020Article
- Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue.Journal of neuroscience methods · 2017Article
- Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system.Nucleic acids research · 2016Article
- Zinc regulates a switch between primary and alternative S18 ribosomal proteins in Mycobacterium tuberculosis.Molecular microbiology · 2015Article
- Effects of Heat Shock and 2,4-D Treatment on Morphological and Physiological Characteristics of Microspores and Microspore-Derived Doubled Haploid Plants inIranian journal of biotechnology · 2015Article
- The homeodomain protein ladybird late regulates synthesis of milk proteins during pregnancy in the tsetse fly (Glossina morsitans).PLoS neglected tropical diseases · 2014Article
- A systems biology approach reveals common metastatic pathways in osteosarcoma.BMC systems biology · 2012Article
- A unique method for isolation and solubilization of proteins after extraction of RNA from tumor tissue using trizol.Journal of biomolecular techniques : JBT · 2011Article
- MudPIT analysis: application to human heart tissue.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage.BioTechniques · 2007Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundResearch with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction, but DNA and protein fractions can also be used for other studies. However, little is known about using TRIzol-extracted proteins in proteomic research, partly because proteins extracted from TRIzol are very resistant to solubilization.
resultsTo facilitate the use of TRIzol-extracted proteins, we first compared the ability of four different common solubilizing reagents to solubilize the TRIzol-extracted proteins from an osteosarcoma cell line, U2-OS. Then we analyzed the solubilized proteins by Surface Enhanced Laser Desorption/Ionization technique (SELDI). The results showed that solubilization of TRIzol-extracted proteins with 9.5 M Urea and 2% CHAPS ([3-[(3-cholamidopropyl)-dimethylammonio]propanesulfonate]) (UREA-CHAPS) was significantly better than the standard 1% SDS in terms of solubilization efficiency and the number of detectable ion peaks. Using three different types of SELDI arrays (CM10, H50, and IMAC-Cu), we demonstrated that peak detection with proteins solubilized by UREA-CHAPS was reproducible (r > 0.9). Further SELDI analysis indicated that the number of ion peaks detected in TRIzol-extracted proteins was comparable to a direct extraction method, suggesting many proteins still remain in the TRIzol protein fraction.
conclusionOur results suggest that UREA-CHAPS performed very well in solubilizing TRIzol-extracted proteins for SELDI applications. Protein fractions left over after TRIzol RNA extraction could be a valuable but neglected source for proteomic or biochemical analysis when additional samples are not available.
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Registered trials
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