ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2006
Motoneurons derived from embryonic stem cells express transcription factors and develop phenotypes characteristic of medial motor column neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 98 citations in OpenAlex.
- The Prospective Regulatory Functions of lncRNAs and Their ceRNA Networks in the Development of Motor Neurons and Associated Diseases.Biomolecules · 2026Review
- Getting in touch with your senses: Mechanisms specifying sensory interneurons in the dorsal spinal cord.WIREs mechanisms of disease · 2021Review
- Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives.Development (Cambridge, England) · 2021Article
- Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules.Military Medical Research · 2020Article
- Engineering geometrical 3-dimensional untethered in vitro neural tissue mimic.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Development, functional organization, and evolution of vertebrate axial motor circuits.Neural development · 2018Review
- Single-injection ex ovo transplantation method for broad spinal cord engraftment of human pluripotent stem cell-derived motor neurons.Journal of neuroscience methods · 2018Article
- Nanotopography regulates motor neuron differentiation of human pluripotent stem cells.Nanoscale · 2018Article
- Microcircuit formation following transplantation of mouse embryonic stem cell-derived neurons in peripheral nerve.Journal of neurophysiology · 2017Article
- Three-Dimensional Organoid System Transplantation Technologies in Future Treatment of Central Nervous System Diseases.Stem cells international · 2017Review
- Development-on-chip: in vitro neural tube patterning with a microfluidic device.Development (Cambridge, England) · 2016Article
- Engineering Stem Cell Organoids.Cell stem cell · 2016Review
- Retinoic Acid-Mediated Regulation of GLI3 Enables Efficient Motoneuron Derivation from Human ESCs in the Absence of Extrinsic SHH Activation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.Nature communications · 2015Article
- Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Established monolayer differentiation of mouse embryonic stem cells generates heterogeneous neocortical-like neurons stalled at a stage equivalent to midcorticogenesis.The Journal of comparative neurology · 2014Article
- Recent developments in cell-based assays and stem cell technologies for botulinum neurotoxin research and drug discovery.Expert review of molecular diagnostics · 2014Review
- Reconstruction of phrenic neuron identity in embryonic stem cell-derived motor neurons.Development (Cambridge, England) · 2014Article
- A stem-cell based bioassay to critically assess the pathology of dysfunctional neuromuscular junctions.PloS one · 2014Article
- Directed midbrain and spinal cord neurogenesis from pluripotent stem cells to model development and disease in a dish.Frontiers in neuroscience · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem (ES) cells differentiate into functional motoneurons when treated with a sonic hedgehog (Shh) agonist and retinoic acid (RA). Whether ES cells can be directed to differentiate into specific subtypes of motoneurons is unknown. We treated embryoid bodies generated from HBG3 ES cells with a Shh agonist and RA for 5 d in culture to induce motoneuron differentiation. Enhanced green fluorescent protein (eGFP) expression was used to identify putative motoneurons, because eGFP is expressed under the control of the Hb9 promoter in HBG3 cells. We found that 96 +/- 0.7% of the differentiated eGFP+ motoneurons expressed Lhx3, a homeobox gene expressed by postmitotic motoneurons in the medial motor column (MMCm), when the treated cells were plated on a neurite-promoting substrate for 5 d. When the treated embryoid bodies were transplanted into stage 17 chick neural tubes, the eGFP+ motoneurons migrated to the MMCm, expressed Lhx3, projected axons to the appropriate target for MMCm motoneurons (i.e., epaxial muscles), and contained synaptic vesicles within intramuscular axonal branches. In ovo and in vitro studies indicated that chemotropic factors emanating from the epaxial muscle and/or surrounding mesenchyme likely guide Lhx3+ motoneurons to their correct target. Finally, whole-cell patch-clamp recordings of transplanted ES cell-derived motoneurons demonstrated that they received synaptic input, elicited repetitive trains of action potentials, and developed passive membrane properties that were similar to host MMCm motoneurons. These results indicate that ES cells can be directed to form subtypes of neurons with specific phenotypic properties.
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