ArticleThe EMBO journal1991
Different mechanisms regulate muscle-specific AChR gamma- and epsilon-subunit gene expression.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 71 citations in OpenAlex.
- Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance.Nature communications · 2025Article
- Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway.Cell & bioscience · 2021Article
- Mechanism of neuromuscular dysfunction in Krabbe disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Systems analysis of transcriptional data provides insights into muscle's biological response to botulinum toxin.Muscle & nerve · 2014Article
- Article
- Expression of pericyte, mesangium and muscle markers in malignant rhabdoid tumor cell lines: differentiation-induction using 5-azacytidine.Cancer science · 2003Article
- Aberrant development of motor axons and neuromuscular synapses in MyoD-null mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Substrate-bound agrin induces expression of acetylcholine receptor epsilon-subunit gene in cultured mammalian muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Local neurotrophic repression of gene transcripts encoding fetal AChRs at rat neuromuscular synapses.The Journal of cell biology · 1995Article
- In vivo and in vitro analysis of electrical activity-dependent expression of muscle acetylcholine receptor genes using adenovirus.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Rapid inhibition of myogenin-driven acetylcholine receptor subunit gene transcription.The EMBO journal · 1994Article
- Upstream sequences of the myogenin gene convey responsiveness to skeletal muscle denervation in transgenic mice.Nucleic acids research · 1993Article
- Neural regulation of muscle acetylcholine receptor epsilon- and alpha-subunit gene promoters in transgenic mice.The Journal of cell biology · 1993Article
- An E box mediates activation and repression of the acetylcholine receptor delta-subunit gene during myogenesis.Molecular and cellular biology · 1993Article
- An 83-nucleotide promoter of the acetylcholine receptor epsilon-subunit gene confers preferential synaptic expression in mouse muscle.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Different E-box regulatory sequences are functionally distinct when placed within the context of the troponin I enhancer.Nucleic acids research · 1992Article
- Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning.Molecular and cellular biology · 1992Article
- Nobel Lecture. Elementary steps in synaptic transmission revealed by currents through single ion channels.The EMBO journal · 1992Review
- The 5'-flanking region of the mouse muscle nicotinic acetylcholine receptor beta subunit gene promotes expression in cultured muscle cells and is activated by MRF4, myogenin and myoD.Nucleic acids research · 1992Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five different subunits, alpha, beta, gamma, delta and epsilon, constitute the acetylcholine receptors from mammalian skeletal muscle. Their corresponding mRNA levels are regulated differentially. In particular, mRNAs encoding the gamma- and epsilon-subunits, which specify two AChR isoforms, show a reciprocal behaviour during synapse formation and maturation. We have isolated 5' flanking sequences of the gamma- and epsilon-subunit genes that confer muscle-specific expression upon transient transfection of primary cultures of rat muscle cells. The gamma-subunit gene fragment contains two adjacent CANNTG sequence motifs that are essential for muscle-specific transcriptional activity suggesting transactivation by helix-loop-helix proteins. The epsilon-subunit gene fragment carries only a single CANNTG consensus motif which is not required for expression in transfected muscle cells. This sequence motif is, however, necessary to repress transcriptional activity in non-muscle cells and thus may control the muscle-specific expression of the epsilon-subunit gene. The results suggest that CANNTG motifs together with their 3' and 5' flanking nucleotides provide binding sites for both activating as well as repressing trans-acting factors. These elements could thus contribute to the muscle-specific expression of AChR subunit genes.
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