ArticleThe Biochemical journal2006
Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 76 citations in OpenAlex.
- Mapping the structural dynamics of red- and blue-emitting beetle luciferases revealed by HDX-MS.Life science alliance · 2026Article
- Bioluminescent enzymatic biosensors: ways to manage their characteristics.Biophysical reviews · 2025Review
- OptimizediScience · 2024Article
- Novel Nanozeolitic Imidazolate Framework (ZIF-8)-Luciferase Biocomposite for Nanosensing Applications.Analytical chemistry · 2023Article
- Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs.Nature cell biology · 2022Article
- A Luciferase Mutant with Improved Brightness and Stability for Whole-Cell Bioluminescent Biosensors and In Vitro Biosensing.Biosensors · 2022Article
- A higher spectral range of beetle bioluminescence with infraluciferin.Frontiers in bioengineering and biotechnology · 2022Article
- Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride.Biotechnology letters · 2021Article
- Non-invasive and high-throughput interrogation of exon-specific isoform expression.Nature cell biology · 2021Article
- SARS-CoV-2 S1 NanoBiT: A nanoluciferase complementation-based biosensor to rapidly probe SARS-CoV-2 receptor recognition.Biosensors & bioelectronics · 2021Article
- Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission.International journal of molecular sciences · 2020Review
- Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours.Nanoscale · 2020Article
- Stem cell delivery to kidney via minimally invasive ultrasound-guided renal artery injection in mice.Scientific reports · 2020Article
- Mutagenesis and Structural Studies Reveal the Basis for the Activity and Stability Properties That Distinguish theBiochemistry · 2019Article
- QuantitativeNeurophotonics · 2019Article
- Development of lipopolyplexes for gene delivery: A comparison of the effects of differing modes of targeting peptide display on the structure and transfection activities of lipopolyplexes.Journal of peptide science : an official publication of the European Peptide Society · 2018Article
- Molecular variation across populations of a widespread North American firefly, Photinus pyralis, reveals that coding changes do not underlie flash color variation or associated visual sensitivity.BMC evolutionary biology · 2018Article
- Structural and dynamical insight into thermally induced functional inactivation of firefly luciferase.PloS one · 2017Article
- Implication of disulfide bridge induced thermal reversibility, structural and functional stability for luciferase.Protein and peptide letters · 2015Article
- Flow-Based Single Cell Deposition for High-Throughput Screening of Protein Libraries.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Firefly luciferase catalyses a two-step reaction, using ATP-Mg2+, firefly luciferin and molecular oxygen as substrates, leading to the efficient emission of yellow-green light. We report the identification of novel luciferase mutants which combine improved pH-tolerance and thermostability and that retain the specific activity of the wild-type enzyme. These were identified by the mutagenesis of solvent-exposed non-conserved hydrophobic amino acids to hydrophilic residues in Photinus pyralis firefly luciferase followed by in vivo activity screening. Mutants F14R, L35Q, V182K, I232K and F465R were found to be the preferred substitutions at the respective positions. The effects of these amino acid replacements are additive, since combination of the five substitutions produced an enzyme with greatly improved pH-tolerance and stability up to 45 degrees C. All mutants, including the mutant with all five substitutions, showed neither a decrease in specific activity relative to the recombinant wild-type enzyme, nor any substantial differences in kinetic constants. It is envisaged that the combined mutant will be superior to wild-type luciferase for many in vitro and in vivo applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.