ArticleNucleic acids research2006
Genome wide distribution of illegitimate recombination events in Kluyveromyces lactis.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing.Synthetic and systems biotechnology · 2023Review
- Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica.Science China. Life sciences · 2021Article
- Gross Chromosomal Rearrangements inInternational journal of molecular sciences · 2020Article
- History of genome editing in yeast.Yeast (Chichester, England) · 2018Review
- Whole-Genome Comparison Reveals Heterogeneous Divergence and Mutation Hotspots in Chloroplast Genome of Eucommia ulmoides Oliver.International journal of molecular sciences · 2018Article
- Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.Synthetic and systems biotechnology · 2017Review
- Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing.Scientific reports · 2016Article
- Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.Microbial cell factories · 2016Article
- Biochemical Characterization of Kat1: a Domesticated hAT-Transposase that Induces DNA Hairpin Formation and MAT-Switching.Scientific reports · 2016Article
- Domesticated transposase Kat1 and its fossil imprints induce sexual differentiation in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Construction of a Kluyveromyces lactis ku80⁻ host strain for recombinant protein production: extracellular secretion of pectin lyase and a streptavidin-pectin lyase chimera.Molecular biotechnology · 2014Article
- Candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics.Current genetics · 2013Article
- Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production.Biotechnology for biofuels · 2013Article
- Maintenance of very long telomeres by recombination in the Kluyveromyces lactis stn1-M1 mutant involves extreme telomeric turnover, telomeric circles, and concerted telomeric amplification.Molecular and cellular biology · 2012Article
- PGASO: A synthetic biology tool for engineering a cellulolytic yeast.Biotechnology for biofuels · 2012Article
- Long telomeres produced by telomerase-resistant recombination are established from a single source and are subject to extreme sequence scrambling.PLoS genetics · 2012Article
- RAS/cyclic AMP and transcription factor Msn2 regulate mating and mating-type switching in the yeast Kluyveromyces lactis.Eukaryotic cell · 2011Article
- Non-homologous end joining plays a key role in transgene concatemer formation in transgenic zebrafish embryos.International journal of biological sciences · 2010Article
- Ume6 is required for the MATa/MATalpha cellular identity and transcriptional silencing in Kluyveromyces lactis.Genetics · 2010Article
- Alpha3, a transposable element that promotes host sexual reproduction.Genes & development · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Illegitimate recombination (IR) is the process by which two DNA molecules not sharing homology to each other are joined. In Kluyveromyces lactis, integration of heterologous DNA occurred very frequently therefore constituting an excellent model organism to study IR. IR was completely dependent on the nonhomologous end-joining (NHEJ) pathway for DNA double strand break (DSB) repair and we detected no other pathways capable of mediating IR. NHEJ was very versatile, capable of repairing both blunt and non-complementary ends efficiently. Mapping the locations of genomic IR-events revealed target site preferences, in which intergenic regions (IGRs) and ribosomal DNA were overrepresented six-fold compared to open reading frames (ORFs). The IGR-events occurred predominantly within transcriptional regulatory regions. In a rad52 mutant strain IR still preferentially occurred at IGRs, indicating that DSBs in ORFs were not primarily repaired by homologous recombination (HR). Introduction of ectopic DSBs resulted in the efficient targeting of IR to these sites, strongly suggesting that IR occurred at spontaneous mitotic DSBs. The targeting efficiency was equal when ectopic breaks were introduced in an ORF or an IGR. We propose that spontaneous DSBs arise more frequently in transcriptional regulatory regions and in rDNA and such DSBs can be mapped by analyzing IR target sites.
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