ArticleMicrobiology (Reading, England)2006
Defects in ex vivo and in vivo growth and sensitivity to osmotic stress of group A Streptococcus caused by interruption of response regulator gene vicR.
Article in Microbiology (Reading, England), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 87 citations in OpenAlex.
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- Elucidation of independently modulated genes inmSystems · 2023Article
- Streptococcus pyogenes TrxSR Two-Component System Regulates Biofilm Production in Acidic Environments.Infection and immunity · 2021Article
- Novel Tyrosine Kinase-Mediated Phosphorylation With Dual Specificity Plays a Key Role in the Modulation ofFrontiers in microbiology · 2021Article
- Characterization of FtsH Essentiality inFrontiers in genetics · 2021Article
- Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group AInfection and immunity · 2020Article
- RidA Proteins Protect against Metabolic Damage by Reactive Intermediates.Microbiology and molecular biology reviews : MMBR · 2020Review
- Another Breaker of the Wall: the Biological Function of the Usp45 Protein of Lactococcus lactis.Applied and environmental microbiology · 2020Article
- Homeostatic control of cell wall hydrolysis by the WalRK two-component signaling pathway ineLife · 2019Article
- Polymorphisms in Regulator of Cov Contribute to the Molecular Pathogenesis of Serotype M28 Group A Streptococcus.The American journal of pathology · 2019Article
- The VraSR regulatory system contributes to virulence in Streptococcus suis via resistance to innate immune defenses.Virulence · 2018Article
- In silico characterisation of the two-component system regulators of Streptococcus pyogenes.PloS one · 2018Article
- Requirement and Synergistic Contribution of Platelet-Activating Factor Acetylhydrolase Sse and Streptolysin S to Inhibition of Neutrophil Recruitment and Systemic Infection by HypervirulentInfection and immunity · 2017Article
- Strain-dependent interactions of Streptococcus gallolyticus subsp. gallolyticus with human blood cells.BMC microbiology · 2017Article
- A novel STK1-targeted small-molecule as an "antibiotic resistance breaker" against multidrug-resistant Staphylococcus aureus.Scientific reports · 2017Article
- Two-component signal transduction systems in oral bacteria.Journal of oral microbiology · 2017Review
- YvqE and CovRS of Group A Streptococcus Play a Pivotal Role in Viability and Phenotypic Adaptations to Multiple Environmental Stresses.PloS one · 2017Article
- Understanding the Streptococcus mutans Cid/Lrg System through CidB Function.Applied and environmental microbiology · 2016Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The regulator VicR of the two-component regulatory system VicRK is essential in several Gram-positive bacteria. However, the authors were able to generate an unconditional vicR insertional mutant of group A Streptococcus. This mutant grew well in rich media but not in non-immune human blood and serum, had attenuated virulence, and was unstable in mice. Complementation of the mutant with vicR expressed in trans restored its phenotype to wild-type. A vicK deletion mutant had a phenotype similar to that of the vicR mutant. Phagocytosis and killing of the vicR mutant were normal, suggesting that VicRK does not regulate processes involved in evasion of host defence. Microarray analysis showed that vicR inactivation down-regulated the transcription of 13 genes, including putative cell wall hydrolase gene pcsB and spy1058-1060, which encode a putative phosphotransferase system enzyme II for carbohydrate transport, and upregulated the expression of five genes, including spy0183 and spy0184, which encode an osmoprotectant transporter OpuA. Consistent with microarray analysis, the vicR mutant took up more of the osmoprotectants betaine and proline and was sensitive to osmotic stress, indicating that vicR inactivation induced osmotic stress and increased susceptibility to osmotic pressure. Additionally, a spy1060 deletion mutant also displayed attenuated virulence. These results suggest that VicRK regulates processes involved in cell wall metabolism, nutrient uptake, and osmotic protection.
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