ArticleBMC veterinary research2006
Serotypes, virulence genes, and PFGE profiles of Escherichia coli isolated from pigs with postweaning diarrhoea in Slovakia.
Article in BMC veterinary research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Association of DiarrheagenicMicroorganisms · 2025Article
- Description of a contemporary pathogenic Escherichia coli isolated from pigs with post-weaning diarrhea in the United States from 2010 to 2023.Veterinary research · 2025Article
- Vaccination with a Live AvirulentAntibiotics (Basel, Switzerland) · 2025Article
- Genomic Characterization of FecalPathogens (Basel, Switzerland) · 2023Article
- An observational field study of porcine post-weaning diarrhea: clinical and microbiological findings, and fecal pH-measurements as a potential diagnostic tool.Porcine health management · 2023Article
- Swine Colibacillosis: Global Epidemiologic and Antimicrobial Scenario.Antibiotics (Basel, Switzerland) · 2023Review
- Vaccination with anAnimals : an open access journal from MDPI · 2022Article
- Antimicrobial Resistance, Serologic and Molecular Characterization ofAntibiotics (Basel, Switzerland) · 2021Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- Association of3 Biotech · 2019Article
- Antimicrobial usage in pig production: Effects on Escherichia coli virulence profiles and antimicrobial resistance.The Onderstepoort journal of veterinary research · 2019Article
- Molecular characterization ofItalian journal of food safety · 2019Article
- Phenotypic and genotypic characterization of enterotoxigenicOpen veterinary journal · 2019Article
- Methodology and application ofJournal of animal science and biotechnology · 2019Review
- Piglet colibacillosis diagnosis based on multiplex polymerase chain reaction and immunohistochemistry of paraffin-embedded tissues.Journal of veterinary science · 2018Article
- Swine Enteric Colibacillosis in Spain: Pathogenic Potential ofFrontiers in microbiology · 2018Article
- Molecular Characterization of Extended-Spectrum β-Lactamase-Producing Multidrug ResistantFrontiers in microbiology · 2018Article
- Microbiome analysis reveals the abundance of bacterial pathogens in Rousettus leschenaultii guano.Scientific reports · 2016Article
- Animal Enterotoxigenic Escherichia coli.EcoSal Plus · 2016Review
- Prevalence of virulence factors in enterotoxigenicPorcine health management · 2016Article
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10 authors.
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Abstract
backgroundPostweaning diarrhoea (PWD) in pigs is usually the main infectious problem of large-scale farms and is responsible for significant losses worldwide. The disease is caused mainly by enterotoxigenic E. coli (ETEC) and Shiga-toxin producing E. coli (STEC). In this study a total of 101 E. coli isolated from pigs with PWD in Slovakia were characterized using phenotypic and genotypic methods.
resultsThese 101 isolates belonged to 40 O:H serotypes. However, 57% of the isolates belonged to only six serotypes (O9:H51, O147:H-, O149:H10, O163:H-, ONT:H-, and ONT:H4), including two new serotypes (O163:H- and ONT:H4) not previously found among porcine ETEC and STEC isolated in other countries. Genes for EAST1, STb, STa, LT and Stx2e toxins were identified in 64%, 46%, 26%, 20%, and 5% of isolates, respectively. PCR showed that 35% of isolates carried genes for F18 colonization factor, and further analyzed by restriction endonuclease revealed that all of them were F18ac. Genes for F4 (K88), F6 (P987), F17, F5 (K99), F41, and intimin (eae gene) adhesins were detected in 19 %, 5%, 3%, 0.9%, 0.9%, and 0.9% of the isolates, respectively. The study of genetic diversity, carried out by PFGE of 46 representative ETEC and STEC isolates, revealed 36 distinct restriction profiles clustered in eight groups. Isolates of the same serotype were placed together in the dendrogram, but high degree of polymorphism among certain serotypes was detected.
conclusionSeropathotype O149:H10 LT/STb/EAST1/F4 (14 isolates) was the most commonly detected followed by O163:H- EAST1/F18 (six isolates), and ONT:H4 STa/STb/Stx2e/F18 (five isolates). Interestingly, this study shows that two new serotypes (O163:H- and ONT:H4) have emerged as pig pathogens in Slovakia. Furthermore, our results show that there is a high genetic variation mainly among ETEC of O149:H10 serotype.
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