ArticleBMC biotechnology2006
Multi-substrate chromosome preparations for high throughput comparative FISH.
Article in BMC biotechnology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Thriving or Withering? Plant Molecular Cytogenetics in the First Quarter of the 21st Century.International journal of molecular sciences · 2025Review
- Multiple origins, one evolutionary trajectory: gradual evolution characterizes distinct lineages of allotetraploid Brachypodium.Genetics · 2023Article
- Cytomolecular Organisation of the Nuclear Genome.International journal of molecular sciences · 2022Article
- To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity inFrontiers in plant science · 2021Article
- Comparatively Barcoded Chromosomes ofInternational journal of molecular sciences · 2019Article
- Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum.Annals of botany · 2018Article
- Resistance of (Frontiers in plant science · 2018Article
- Similarities and differences in the nuclear genome organization within Pooideae species revealed by comparative genomic in situ hybridization (GISH).Journal of applied genetics · 2017Article
- Article
- Characterization of morphology and resistance to Blumeria graminis of winter triticale monosomic addition lines with chromosome 2D of Aegilops tauschii.Plant cell reports · 2016Article
- Allocation of the S-genome chromosomes of Aegilops variabilis Eig. carrying powdery mildew resistance in triticale (× Triticosecale Wittmack).Protoplasma · 2016Article
- Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei.PloS one · 2016Article
- Effective transfer of chromosomes carrying leaf rust resistance genes from Aegilops tauschii Coss. into hexaploid triticale (X Triticosecale Witt.) using Ae. tauschii × Secale cereale amphiploid forms.Journal of applied genetics · 2015Article
- Reconstructing the Evolution of Brachypodium Genomes Using Comparative Chromosome Painting.PloS one · 2014Article
- Insight into the karyotype evolution of brachypodium species using comparative chromosome barcoding.PloS one · 2014Article
- Introgression of A- and B-genome of tetraploid triticale chromatin into tetraploid rye.Journal of applied genetics · 2013Article
- Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum.Cellular & molecular biology letters · 2012Article
- Compact genomes and complex evolution in the genus Brachypodium.Chromosoma · 2011Article
- Comparative cytogenetic analysis of the genomes of the model grass Brachypodium distachyon and its close relatives.Annals of botany · 2009Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods.
resultsThe utility and application of multi-colour, multi-substrate FISH is illustrated by the simultaneous physical mapping of retrotransposon sequences to three species of Avena, and single locus BAC (bacterial artificial chromosome) clones and rDNA probes to three species of Brachypodium, demonstrating how this would enable better understanding of complex phylogenetic relationships among some of the species belonging to these two genera.
conclusionThe results show that use of multi-substrate chromosome preparations significantly increases the utility of FISH in comparative analyses of the distribution and abundance of chromosomal sequences in closely related plant species.
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