ArticleJournal of bacteriology2006
Cross-regulation of the Bacillus subtilis glnRA and tnrA genes provides evidence for DNA binding site discrimination by GlnR and TnrA.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- The effect of nitrogen availability and forms on the degradation pathway of lignocellulose by Bacillus subtilis.Bioprocess and biosystems engineering · 2026Article
- Glutamine synthetase and GlnR regulate nitrogen metabolism inApplied and environmental microbiology · 2023Article
- Understanding and application of Bacillus nitrogen regulation: A synthetic biology perspective.Journal of advanced research · 2023Review
- Genome-wide mapping of GlnR-binding sites reveals the global regulatory role of GlnR in controlling the metabolism of nitrogen and carbon in Paenibacillus polymyxa WLY78.BMC genomics · 2023Article
- Alanine synthesized by alanine dehydrogenase enables ammonium-tolerant nitrogen fixation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Role of GlnR in Controlling Expression of Nitrogen Metabolism Genes inJournal of bacteriology · 2020Article
- GlnR Negatively Regulates Glutamate-Dependent Acid Resistance in Lactobacillus brevis.Applied and environmental microbiology · 2020Article
- Bacillus velezensis LG37: transcriptome profiling and functional verification of GlnK and MnrA in ammonia assimilation.BMC genomics · 2020Article
- Positive and negative regulation of transferred nif genes mediated by indigenous GlnR in Gram-positive Paenibacillus polymyxa.PLoS genetics · 2018Article
- Revisiting the in vivo GlnR-binding sites at the genome scale in Bacillus subtilis.BMC research notes · 2017Article
- The GlnR Regulon in Streptococcus mutans Is Differentially Regulated by GlnR and PmrA.PloS one · 2016Article
- Genome-wide mapping of TnrA-binding sites provides new insights into the TnrA regulon in Bacillus subtilis.MicrobiologyOpen · 2015Article
- Lessons from the modular organization of the transcriptional regulatory network of Bacillus subtilis.BMC systems biology · 2013Article
- Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis.Journal of bacteriology · 2013Article
- Comparative genome analysis of central nitrogen metabolism and its control by GlnR in the class Bacilli.BMC genomics · 2012Article
- At the crossroads of bacterial metabolism and virulence factor synthesis in Staphylococci.Microbiology and molecular biology reviews : MMBR · 2009Review
- Novel trans-Acting Bacillus subtilis glnA mutations that derepress glnRA expression.Journal of bacteriology · 2009Article
- Switching control of expression of ptsG from the Mlc regulon to the NagC regulon.Journal of bacteriology · 2008Article
- Site-specific contributions of glutamine-dependent regulator GlnR and GlnR-regulated genes to virulence of Streptococcus pneumoniae.Infection and immunity · 2008Article
- Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Two Bacillus subtilis transcriptional factors, TnrA and GlnR, regulate gene expression in response to changes in nitrogen availability. These two proteins have similar amino acid sequences in their DNA binding domains and bind to DNA sites (GlnR/TnrA sites) that have the same consensus sequence. Expression of the tnrA gene was found to be activated by TnrA and repressed by GlnR. Mutational analysis demonstrated that a GlnR/TnrA site which lies immediately upstream of the -35 region of the tnrA promoter is required for regulation of tnrA expression by both GlnR and TnrA. Expression of the glnRA operon, which contains two GlnR/TnrA binding sites (glnRAo1 and glnRAo2) in its promoter region, is repressed by both GlnR and TnrA. The glnRAo2 site, which overlaps the -35 region of the glnRA promoter, was shown to be required for regulation by both GlnR and TnrA, while the glnRAo1 site which lies upstream of the -35 promoter region is only involved in GlnR-mediated regulation. Examination of TnrA binding to tnrA and glnRA promoter DNA in gel mobility shift experiments showed that TnrA bound with an equilibrium dissociation binding constant of 55 nM to the GlnR/TnrA site in the tnrA promoter region, while the affinities of TnrA for the two GlnR/TnrA sites in the glnRA promoter region were greater than 3 muM. These results demonstrate that GlnR and TnrA cross-regulate each other's expression and that there are differences in their DNA-binding specificities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.