ArticleJournal of bacteriology2006
Mutations in DivL and CckA rescue a divJ null mutant of Caulobacter crescentus by reducing the activity of CtrA.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 39 citations in OpenAlex.
- Pst and Tat Systems Are Essential forMicroorganisms · 2026Article
- TheJournal of bacteriology · 2024Article
- ATP-responsive biomolecular condensates tune bacterial kinase signaling.Science advances · 2022Article
- Analysis ofJournal of bacteriology · 2019Article
- Sensory domain of the cell cycle kinase CckA regulates the differential DNA binding of the master regulator CtrA in Caulobacter crescentus.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Article
- The Protease ClpXP and the PAS Domain Protein DivL Regulate CtrA and Gene Transfer Agent Production in Rhodobacter capsulatus.Applied and environmental microbiology · 2018Article
- In-phase oscillation of global regulons is orchestrated by a pole-specific organizer.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- VAP, a Versatile Access Point for the Endoplasmic Reticulum: Review and analysis of FFAT-like motifs in the VAPome.Biochimica et biophysica acta · 2016Review
- Cell fate regulation governed by a repurposed bacterial histidine kinase.PLoS biology · 2014Article
- Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems.Molecular microbiology · 2014Article
- Comparative genomics of cultured and uncultured strains suggests genes essential for free-living growth of Liberibacter.PloS one · 2014Article
- Spatial perturbation with synthetic protein scaffold reveals robustness of asymmetric cell division.Journal of biomedical science and engineering · 2013Article
- Potential role of a bistable histidine kinase switch in the asymmetric division cycle of Caulobacter crescentus.PLoS computational biology · 2013Article
- Coordination of division and development influences complex multicellular behavior in Agrobacterium tumefaciens.PloS one · 2013Article
- Polarity and cell fate asymmetry in Caulobacter crescentus.Current opinion in microbiology · 2012Review
- The scaffolding and signalling functions of a localization factor impact polar development.Molecular microbiology · 2012Article
- A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentus.Developmental cell · 2011Article
- Single-gene tuning of Caulobacter cell cycle period and noise, swarming motility, and surface adhesion.Molecular systems biology · 2010Article
- Cell pole-specific activation of a critical bacterial cell cycle kinase.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Getting in the loop: regulation of development in Caulobacter crescentus.Microbiology and molecular biology reviews : MMBR · 2010Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Polar development and cell division in Caulobacter crescentus are controlled and coordinated by multiple signal transduction proteins. divJ encodes a histidine kinase. A null mutation in divJ results in a reduced growth rate, cell filamentation, and mislocalized stalks. Suppressor analysis of divJ identified mutations in genes encoding the tyrosine kinase (divL) and the histidine kinase (cckA). The divL and cckA suppressor alleles all have single amino acid substitutions, some of which confer a temperature-sensitive phenotype, particularly in a wild-type background. Analysis of transcription levels from several positively regulated CtrA-dependent promoters reveals high expression in the divJ mutant, suggesting that DivJ normally serves to reduce CtrA activity. The divL and cckA suppressors reduce the amount of transcription from promoters positively regulated by CtrA, indicating that the mutations in divL and cckA are suppressing the defects of the divJ mutant by reducing the abnormally high level of CtrA activity. Immunoblotting showed no major perturbations in the CtrA protein level in any of these strains, suggesting that the high amount of CtrA activity seen in the divJ mutant and the reduced amount of activity in the suppressors are regulated at the level of activation and not transcription, translation, or degradation. In vivo phosphorylation assays confirmed that divJ mutants have elevated levels of CtrA phosphorylation and that this level is reduced in the suppressors with mutations in divL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.