ArticleFree radical biology & medicine2006
Effect of heme and heme oxygenase-1 on vascular endothelial growth factor synthesis and angiogenic potency of human keratinocytes.
Article in Free radical biology & medicine, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 89 citations in OpenAlex.
- Haem arginate infusion stimulates haem oxygenase-1 expression in healthy subjects.British journal of pharmacology · 2010Trial
- Review
- Effects of Dietary Ferroporphyrin Supplementation on Growth Performance, Antioxidant Capacity, Immune Response, and Oxygen-Carrying Capacity in Gibel Carp (Animals : an open access journal from MDPI · 2024Article
- Ozone therapy for skin diseases: Cellular and molecular mechanisms.International wound journal · 2023Review
- Ozonated saline intradermal injection: promising therapy for accelerated cutaneous wound healing in diabetic rats.Frontiers in veterinary science · 2023Article
- Argon Mitigates Impaired Wound Healing Process and Enhances Wound HealingTheranostics · 2019Article
- Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2.Oxidative medicine and cellular longevity · 2018Article
- Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways.Scientific reports · 2016Article
- Myoblast-conditioned media improve regeneration and revascularization of ischemic muscles in diabetic mice.Stem cell research & therapy · 2015Article
- Non-thermal plasma activates human keratinocytes by stimulation of antioxidant and phase II pathways.The Journal of biological chemistry · 2015Article
- The Role of miR-378a in Metabolism, Angiogenesis, and Muscle Biology.International journal of endocrinology · 2015Review
- Hypoxia-responsive microRNA-101 promotes angiogenesis via heme oxygenase-1/vascular endothelial growth factor axis by targeting cullin 3.Antioxidants & redox signaling · 2014Article
- Unspliced X-box-binding protein 1 (XBP1) protects endothelial cells from oxidative stress through interaction with histone deacetylase 3.The Journal of biological chemistry · 2014Article
- Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: nuclear HO-1 promotes vascular endothelial growth factor secretion.International journal of oncology · 2013Article
- The role of vascular endothelial growth factor in fractional laser resurfacing with the carbon dioxide laser.Lasers in medical science · 2012Article
- Heme oxygenase-1 inhibits myoblast differentiation by targeting myomirs.Antioxidants & redox signaling · 2012Article
- Empowering adult stem cells for myocardial regeneration.Circulation research · 2011Review
- Increased levels of plasma haemoxygenase-1 in prostate cancer.Prostate cancer and prostatic diseases · 2011Article
- Comparison of EGF with VEGF non-viral gene therapy for cutaneous wound healing of streptozotocin diabetic mice.Diabetes & metabolism journal · 2011Article
- A new technique of ex vivo gene delivery of VEGF to wounds using genetically modified skin particles promotes wound angiogenesis.Journal of the American College of Surgeons · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSkin injury leads to the release of heme, a potent prooxidant which is degraded by heme oxygenase-1 (HO-1) to carbon monoxide, iron, and biliverdin, subsequently reduced to bilirubin. Recently the involvement of HO-1 in angiogenesis has been shown; however, the role of heme and HO-1 in wound healing angiogenesis has not been yet investigated.
resultsTreatment of HaCaT keratinocytes with hemin (heme chloride) induced HO-1 expression and activity. The effect of heme on vascular endothelial growth factor (VEGF) synthesis is variable: induction is significant after a short, 6 h treatment with heme, while longer stimulation may attenuate its production. The involvement of HO-1 in VEGF synthesis was confirmed by inhibition of VEGF expression by SnPPIX, a blocker of HO activity and by attenuation of HO-1 mRNA expression with specific siRNA. Importantly, induction of HO-1 by hemin was able to overcome the inhibitory effect of high glucose on VEGF synthesis. Moreover, HO-1 expression was also induced in keratinocytes cultured in hypoxia, with concomitant augmentation of VEGF production, which was further potentiated by hemin stimulation. Accordingly, conditioned media from keratinocytes overexpressing HO-1 enhanced endothelial cell proliferation and augmented formation of capillaries in angiogenic assay in vitro.
conclusionsHO-1 is involved in hemin-induced VEGF expression in HaCaT and may play a role in hypoxic regulation of this protein. HO-1 overexpression may be beneficial in restoring the proper synthesis of VEGF disturbed in diabetic conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.