ArticleJournal of fluorescence2006
3D particle tracking on a two-photon microscope.
Article in Journal of fluorescence, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Localization of single molecules with structured illumination and structured detection.Light, science & applications · 2025Article
- 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.Nature communications · 2024Article
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- A common framework for single-molecule localization using sequential structured illumination.Biophysical reports · 2022Article
- Interferometric fluorescence cross correlation spectroscopy.PloS one · 2019Article
- Tracking single fluorescent particles in three dimensions via extremum seeking.Biomedical optics express · 2016Article
- Two-Photon Fluorescence Tracking of Colloidal Clusters.Journal of fluorescence · 2016Article
- Electrically tunable lens speeds up 3D orbital tracking.Biomedical optics express · 2015Article
- Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.The Journal of cell biology · 2015Article
- Advanced fluorescence microscopy methods for the real-time study of transcription and chromatin dynamics.Transcription · 2014Review
- Fluorescence techniques to study lipid dynamics.Cold Spring Harbor perspectives in biology · 2011Review
- Parallax: high accuracy three-dimensional single molecule tracking using split images.Nano letters · 2009Article
- Slow stress propagation in adherent cells.Biophysical journal · 2008Article
- Micro and Nano-Scale Technologies for Cell Mechanics.NanobiomedicineReview
- Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy.Journal of biomedical opticsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 3D single-particle-tracking (SPT) system was developed based on two-photon excitation fluorescence microscopy that can track the motion of particles in three dimensions over a range of 100 mum and with a bandwidth up to 30 Hz. We have implemented two different techniques employing feedback control. The first technique scans a small volume around a particle to build up a volumetric image that is then used to determine the particle's position. The second technique scans only a single plane but utilizes optical aberrations that have been introduced into the optical system that break the axial symmetry of the point spread function and serve as an indicator of the particle's axial position. We verified the performance of the instrument by tracking particles in well-characterized models systems. We then studied the 3D viscoelastic mechanical response of 293 kidney cells using the techniques. Force was applied to the cells, by using a magnetic manipulator, onto the paramagnetic spheres attached to the cell via cellular integrin receptors. The deformation of the cytoskeleton was monitored by following the motion of nearby attached fluorescent polystyrene spheres. We showed that planar stress produces strain in all three dimensions, demonstrating that the 3D motion of the cell is required to fully model cellular mechanical responses.
Identifiers
16544202What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.