ArticleThe Journal of cell biology1991
Phospholipase C release of basic fibroblast growth factor from human bone marrow cultures as a biologically active complex with a phosphatidylinositol-anchored heparan sulfate proteoglycan.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 144 citations in OpenAlex.
- (Patho)Physiology of Glycosylphosphatidylinositol-Anchored Proteins I: Localization at Plasma Membranes and Extracellular Compartments.Biomolecules · 2023Review
- Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations.Stem cells and development · 2015Article
- An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation.Journal of visualized experiments : JoVE · 2015Article
- The interrelationship between bone and fat: from cellular see-saw to endocrine reciprocity.Cellular and molecular life sciences : CMLS · 2013Review
- Proteoglycan 4: a dynamic regulator of skeletogenesis and parathyroid hormone skeletal anabolism.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
- Dual FGF-2 and intergrin alpha5beta1 signaling mediate GRAF-induced RhoA inactivation in a model of breast cancer dormancy.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2009Article
- Loss of glypican-3 function causes growth factor-dependent defects in cardiac and coronary vascular development.Developmental biology · 2009Article
- Article
- Mammalian and Drosophila cells adhere to the laminin alpha4 LG4 domain through syndecans, but not glypicans.The Biochemical journal · 2004Article
- Plasminogen activators direct reorganization of the liver lobule after acute injury.The American journal of pathology · 2001Article
- Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation.The Journal of clinical investigation · 2000Article
- Plasminogen deficiency leads to impaired remodeling after a toxic injury to the liver.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Inflammatory mediators regulate cathepsin S in macrophages and microglia: A role in attenuating heparan sulfate interactions.Molecular medicine (Cambridge, Mass.) · 1999Article
- Murine embryonic stem cells without pig-a gene activity are competent for hematopoiesis with the PNH phenotype but not for clonal expansion.The Journal of clinical investigation · 1997Article
- Involvement of heparan sulfate and related molecules in sequestration and growth promoting activity of fibroblast growth factor.Cancer metastasis reviews · 1996Review
- Release of GPI-anchored membrane proteins by a cell-associated GPI-specific phospholipase D.The EMBO journal · 1994Article
- Accumulation of PDGF B and cell-binding forms of PDGF A in the extracellular matrix.The Journal of cell biology · 1993Article
- Isolation and purification of proteoglycans.Experientia · 1993Review
- Cytokines and proteoglycans.Experientia · 1993Review
- Heat shock induces the release of fibroblast growth factor 1 from NIH 3T3 cells.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Basic fibroblast growth factor (bFGF) is a potent mitogen for human bone marrow stromal cells and stimulates haematopoiesis in vitro. We report here that primary human bone marrow cultures contain bFGF and express heparin-like bFGF binding sites on the cell surface and in the extracellular matrix (ECM). bFGF bound predominantly to a 200-kD cell surface heparan sulfate proteoglycan (HSPG), which was also found in conditioned medium. bFGF was released from bone marrow cultures by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) and, less efficiently, by plasmin. Solubilized bFGF was found as a complex with the 200-kD HSPG. The complex was biologically active as shown by its ability to stimulate plasminogen activator production in bovine aortic endothelial cells. bFGF-HSPG complexes of bovine endothelial cells, however, were not released by PI-PLC. While only trace amounts of the bFGF-binding 200-kD HSPG were released spontaneously from bone marrow cultures, incubation with PI-PLC solubilized almost all of the 200-kD HSPG. The HSPG could be metabolically labeled with ethanolamine or palmitate, which was partially removed by treatment with PI-PLC. These findings indicate linkage of the HSPG to the cell surface via a phosphatidylinositol anchor. Plasmin released the 200-kD HSPG less efficiently than PI-PLC. We conclude that HSPGs of human bone marrow serve as a reservoir for bFGF, from which it can be released in a biologically active form via a dual mechanism; one involving a putative endogenous phospholipase, the other involving the proteolytic cascade of plasminogen activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.