ArticleRNA (New York, N.Y.)2006
Functional links between the Prp19-associated complex, U4/U6 biogenesis, and spliceosome recycling.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- GPATCH11 ortholog Sap34 regulates pre-mRNA splicing by interacting with early spliceosomal complexes in Schizosaccharomyces pombe.Scientific reports · 2026Article
- Analysis of the mechanism of tripropylamine-enhanced lycopene accumulation in Blakeslea trispora through transcriptome.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- The PRP19 Ubiquitin Ligase, Standing at the Cross-Roads of mRNA Processing and Genome Stability.Cancers · 2022Review
- The ATR-WEE1 kinase module inhibits the MAC complex to regulate replication stress response.Nucleic acids research · 2021Article
- RNA Binding Proteins and Genome Integrity.International journal of molecular sciences · 2017Review
- Roles of RNA-Binding Proteins in DNA Damage Response.International journal of molecular sciences · 2016Review
- The PSO4 protein complex associates with replication protein A (RPA) and modulates the activation of ataxia telangiectasia-mutated and Rad3-related (ATR).The Journal of biological chemistry · 2014Article
- PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.Molecular cell · 2014Article
- Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis.Molecular and cellular biology · 2014Article
- Structural basis for dual roles of Aar2p in U5 snRNP assembly.Genes & development · 2013Article
- Decoding ubiquitin for mitosis.Genes & cancer · 2012Article
- The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator.Frontiers in plant science · 2012Article
- MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.PloS one · 2012Article
- The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing.Biochemical Society transactions · 2010Review
- The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.Genes & development · 2010Article
- Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation.RNA (New York, N.Y.) · 2009Article
- The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing.Nucleic acids research · 2009Article
- Deciphering the ubiquitin-mediated pathway in apicomplexan parasites: a potential strategy to interfere with parasite virulence.PloS one · 2008Article
- Spliceosomal small nuclear ribonucleoprotein particles repeatedly cycle through Cajal bodies.Molecular biology of the cell · 2008Article
- A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Prp19-associated complex, consisting of at least eight protein components, is involved in spliceosome activation by specifying the interaction of U5 and U6 with pre-mRNA for their stable association with the spliceosome after U4 dissociation. We show here that yeast cells depleted of one or two of the Prp19-associated components, accumulate the free form of U4. In NTC25-deleted cells, the level of U6 was also reduced. Extracts prepared from NTC25-deleted cells contained neither free U4 nor U6 and were ineffective in spliceosome recycling in the in vitro splicing reaction. Overexpression of U6 partially rescued the temperature-sensitive growth defect and decreased the relative amount of free U4 in NTC25-deleted cells, indicating that the accumulation of free U4 was a consequence of insufficient amounts of U6 snRNA. Extracts prepared from U6-overproducing NTC25-deleted cells containing free-form U6 were capable of spliceosome recycling, suggesting a role of free U6 RNP in spliceosome recycling. Our results demonstrate that in addition to direct participation in spliceosome activation, the Prp19-associated complex has an indirect role in spliceosome recycling through affecting the biogenesis of U4/U6 snRNP in the in vivo splicing reaction.
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