ArticleNucleic acids research2006
Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Plasmid-DNA Delivery by Covalently Functionalized PEI-SPIONs as a Potential 'Magnetofection' Agent.Molecules (Basel, Switzerland) · 2022Article
- Magnetofection In Vivo by Nanomagnetic Carriers Systemically Administered into the Bloodstream.Pharmaceutics · 2021Review
- Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape.Nanomaterials (Basel, Switzerland) · 2020Review
- Progressive lysosomal membrane permeabilization induced by iron oxide nanoparticles drives hepatic cell autophagy and apoptosis.Nano convergence · 2020Article
- A Critical Review on Selected External Physical Cues and Modulation of Cell Behavior: Magnetic Nanoparticles, Non-thermal Plasma and Lasers.Journal of functional biomaterials · 2018Review
- Magnetofection Mediated Transient NANOG Overexpression Enhances Proliferation and Myogenic Differentiation of Human Hair Follicle Derived Mesenchymal Stem Cells.Bioconjugate chemistry · 2015Article
- Nanotechnology in bone tissue engineering.Nanomedicine : nanotechnology, biology, and medicine · 2015Review
- Iron oxide nanoparticles for magnetically-guided and magnetically-responsive drug delivery.International journal of molecular sciences · 2015Review
- Tumour cell labelling by magnetic nanoparticles with determination of intracellular iron content and spatial distribution of the intracellular iron.International journal of molecular sciences · 2013Article
- Handling of iron oxide and silver nanoparticles by astrocytes.Neurochemical research · 2013Review
- Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field.Journal of genetics · 2012Article
- Magnetically enhanced nucleic acid delivery. Ten years of magnetofection-progress and prospects.Advanced drug delivery reviews · 2011Review
- Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity.Journal of nanobiotechnology · 2011Article
- The application of nanoparticles in gene therapy and magnetic resonance imaging.Microscopy research and technique · 2011Review
- Magnetic nanoparticles as targeted delivery systems in oncology.Radiology and oncology · 2011Article
- Application of magnetic nanoparticles to gene delivery.International journal of molecular sciences · 2011Review
- Gene transfer: how can the biological barriers be overcome?The Journal of membrane biology · 2010Article
- Increased cellular uptake of biocompatible superparamagnetic iron oxide nanoparticles into malignant cells by an external magnetic field.The Journal of membrane biology · 2010Article
- Evaluation of the magnetic field requirements for nanomagnetic gene transfection.Nano reviews · 2010Article
- A novel magnetic crystal-lipid nanostructure for magnetically guided in vivo gene delivery.Nature nanotechnology · 2009Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New approaches to increase the efficiency of non-viral gene delivery are still required. Here we report a simple approach that enhances gene delivery using permanent and pulsating magnetic fields. DNA plasmids and novel DNA fragments (PCR products) containing sequence encoding for green fluorescent protein were coupled to polyethylenimine coated superparamagnetic nanoparticles (SPIONs). The complexes were added to cells that were subsequently exposed to permanent and pulsating magnetic fields. Presence of these magnetic fields significantly increased the transfection efficiency 40 times more than in cells not exposed to the magnetic field. The transfection efficiency was highest when the nanoparticles were sedimented on the permanent magnet before the application of the pulsating field, both for small (50 nm) and large (200-250 nm) nanoparticles. The highly efficient gene transfer already within 5 min shows that this technique is a powerful tool for future in vivo studies, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.
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Registered trials
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