ArticleBMC microbiology2006
Microarray based comparison of two Escherichia coli O157:H7 lineages.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 50 citations in OpenAlex.
- Ultrasensitive and rapid count of Escherichia coli using magnetic nanoparticle probe under dark-field microscope.BMC microbiology · 2018Article
- Microscopy and genomic analysis of Mycoplasma parvum strain Indiana.Veterinary research · 2014Article
- Identification and characterization of a peculiar vtx2-converting phage frequently present in verocytotoxin-producing Escherichia coli O157 isolated from human infections.Infection and immunity · 2014Article
- Variation in stress resistance patterns among stx genotypes and genetic lineages of shiga toxin-producing Escherichia coli O157.Applied and environmental microbiology · 2012Article
- Evolutionary and functional insights into Leishmania META1: evidence for lateral gene transfer and a role for META1 in secretion.BMC evolutionary biology · 2011Article
- Study of polymorphisms in tir, eae and tccP2 genes in enterohaemorrhagic and enteropathogenic Escherichia coli of serogroup O26.BMC microbiology · 2011Article
- Genome signatures of Escherichia coli O157:H7 isolates from the bovine host reservoir.Applied and environmental microbiology · 2011Article
- Multivariate analyses revealed distinctive features differentiating human and cattle isolates of Shiga toxin-producing Escherichia coli O157 in Japan.Journal of clinical microbiology · 2011Article
- Transcriptomic analysis for genetic mechanisms of the factors related to biofilm formation in Escherichia coli O157:H7.Current microbiology · 2011Article
- Role of RpoS in virulence of pathogens.Infection and immunity · 2010Review
- Lineage and host source are both correlated with levels of Shiga toxin 2 production by Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2010Article
- Escherichia coli O157:H7 strain origin, lineage, and Shiga toxin 2 expression affect colonization of cattle.Applied and environmental microbiology · 2009Article
- In silico genomic analyses reveal three distinct lineages of Escherichia coli O157:H7, one of which is associated with hyper-virulence.BMC genomics · 2009Article
- Genomic regions conserved in lineage II Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2009Article
- Enterohemorrhagic Escherichia coli O157:H7 gene expression profiling in response to growth in the presence of host epithelia.PloS one · 2009Article
- Anaerobic conditions promote expression of Sfp fimbriae and adherence of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM to human intestinal epithelial cells.Applied and environmental microbiology · 2008Article
- Comparative transcriptome analysis of Listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall, and stress response.Applied and environmental microbiology · 2007Article
- Genome evolution in major Escherichia coli O157:H7 lineages.BMC genomics · 2007Article
- Expression of putative virulence factors of Escherichia coli O157:H7 differs in bovine and human infections.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious research has identified the potential for the existence of two separate lineages of Escherichia coli O157:H7. Clinical isolates tended to cluster primarily within one of these two lineages. To determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening.
resultsUsing a 610 gene microarray, designed against the E. coli O157 EDL 933 transcriptome, targeting primarily virulence systems, we chose 3 representative Lineage I isolates (LI groups mostly clinical isolates) and 3 representative Lineage II isolates (LII groups mostly bovine isolates). Using standard dye swap experimental designs, statistically different expression (P < 0.05) of 73 genes between the two lineages was revealed. Result highlights indicate that under in vitro anaerobic growth conditions, there is up-regulation of stx2b, ureD, curli (csgAFEG), and stress related genes (hslJ, cspG, ibpB, ibpA) in Lineage I, which may contribute to enhanced virulence or transmission potential. Lineage II exhibits significant up-regulation of type III secretion apparatus, LPS, and flagella related transcripts.
conclusionThese results give insight into comparative regulation of virulence genes as well as providing directions for future research. Ultimately, evaluating the expression of key virulence factors among different E. coli O157 isolates has inherent value and the interpretation of such expression data will continue to evolve as our understanding of virulence, pathogenesis and transmission improves.
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