ArticleJournal of virology2006
Selection of novel vesicular stomatitis virus glycoprotein variants from a peptide insertion library for enhanced purification of retroviral and lentiviral vectors.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Improved Manufacturing Methods of Extracellular Vesicles Pseudotyped with the Vesicular Stomatitis Virus Glycoprotein.Molecular biotechnology · 2024Article
- Nuclear dengue virus NS5 antagonizes expression of PAF1-dependent immune response genes.PLoS pathogens · 2021Article
- Tagging and Capturing of Lentiviral Vectors Using Short RNAs.International journal of molecular sciences · 2021Article
- Transient light-activated gene expression in Chinese hamster ovary cells.BMC biotechnology · 2021Article
- Experimental Evolution Generates Novel Oncolytic Vesicular Stomatitis Viruses with Improved Replication in Virus-Resistant Pancreatic Cancer Cells.Journal of virology · 2020Article
- Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell.Molecular therapy. Methods & clinical development · 2018Article
- Novel GP64 envelope variants for improved delivery to human airway epithelial cells.Gene therapy · 2017Article
- cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering.Nature communications · 2015Article
- Single-cell western blotting.Nature methods · 2014Article
- Characteristics of oncolytic vesicular stomatitis virus displaying tumor-targeting ligands.Journal of virology · 2013Article
- Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors.Gene therapy · 2013Article
- Astrocytes regulate adult hippocampal neurogenesis through ephrin-B signaling.Nature neuroscience · 2012Article
- Directed evolution of adeno-associated virus for enhanced gene delivery and gene targeting in human pluripotent stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Akt increases sox2 expression in adult hippocampal neural progenitor cells, but increased sox2 does not promote proliferation.Stem cells and development · 2011Article
- An evolved adeno-associated viral variant enhances gene delivery and gene targeting in neural stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- HIV evades RNA interference directed at TAR by an indirect compensatory mechanism.Cell host & microbe · 2008Article
- Molecular engineering of viral gene delivery vehicles.Annual review of biomedical engineering · 2008Review
- Gene delivery by lentivirus vectors.Molecular biotechnology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The introduction of new features or functions that are not present in an original protein is a significant challenge in protein engineering. For example, modifications to vesicular stomatitis virus glycoprotein (VSV-G), which is commonly used to pseudotype retroviral and lentiviral vectors for gene delivery, have been hindered by a lack of structural knowledge of the protein. We have developed a transposon-based approach that randomly incorporates designed polypeptides throughout a protein to generate saturated insertion libraries and a subsequent high-throughput selection process in mammalian cells that enables the identification of optimal insertion sites for a novel designed functionality. This method was applied to VSV-G in order to construct a comprehensive library of mutants whose combined members have a His6 tag inserted at likely every site in the original protein sequence. Selecting the library via iterative retroviral infections of mammalian cells led to the identification of several VSV-G-His6 variants that were able to package high-titer viral vectors and could be purified by Ni-nitrilotriacetic acid affinity chromatography. Column purification of vectors reduced protein and DNA impurities more than 5,000-fold and 14,000-fold, respectively, from the viral supernatant. This substantially improved purity elicited a weaker immune response in the brain, without altering the infectivity or tropism from wild-type VSV-G-pseudotyped vectors. This work applies a powerful new tool for protein engineering to construct novel viral envelope variants that can greatly improve the safety and use of retroviral and lentiviral vectors for clinical gene therapy. Furthermore, this approach of library generation and selection can readily be extended to other challenges in protein engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.