ArticleProceedings of the National Academy of Sciences of the United States of America2006
cAMP regulates plasma membrane vacuolar-type H+-ATPase assembly and activity in blowfly salivary glands.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Dynamics of release and activity of select neuropeptides post-bloodmeal in the female mosquito, Aedes aegypti.The Journal of experimental biology · 2025Article
- The V-type HProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Tender love and disassembly: How a TLDc domain protein breaks the V-ATPase.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- The HAmerican journal of physiology. Cell physiology · 2021Review
- Regulation and function of V-ATPases in physiology and disease.Biochimica et biophysica acta. Biomembranes · 2020Review
- Cilostazol restores autophagy flux in bafilomycin A1-treated, cultured cortical astrocytes through lysosomal reacidification: roles of PKA, zinc and metallothionein 3.Scientific reports · 2020Article
- Review
- Cysteine protease cathepsins in cardiovascular disease: from basic research to clinical trials.Nature reviews. Cardiology · 2018Review
- Correlative Gene Expression to Protective Seroconversion in Rift Valley Fever Vaccinates.PloS one · 2016Article
- Fluid absorption in the isolated midgut of adult female yellow fever mosquitoes (Aedes aegypti).The Journal of experimental biology · 2015Article
- Feeding induces translocation of vacuolar proton ATPase and pendrin to the membrane of leopard shark (Triakis semifasciata) mitochondrion-rich gill cells.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2014Article
- Regulation of luminal acidification by the V-ATPase.Physiology (Bethesda, Md.) · 2013Review
- Separate roles of PKA and EPAC in renal function unraveled by the optogenetic control of cAMP levels in vivo.Journal of cell science · 2013Article
- Targeting reversible disassembly as a mechanism of controlling V-ATPase activity.Current protein & peptide science · 2012Review
- Transcellular and paracellular pathways of transepithelial fluid secretion in Malpighian (renal) tubules of the yellow fever mosquito Aedes aegypti.Acta physiologica (Oxford, England) · 2011Review
- Deletion of the pH sensor GPR4 decreases renal acid excretion.Journal of the American Society of Nephrology : JASN · 2010Article
- PKA regulates vacuolar H+-ATPase localization and activity via direct phosphorylation of the a subunit in kidney cells.The Journal of biological chemistry · 2010Article
- Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase.The EMBO journal · 2010Article
- Vacuolar H+-ATPase apical accumulation in kidney intercalated cells is regulated by PKA and AMP-activated protein kinase.American journal of physiology. Renal physiology · 2010Article
- Vacuolar-type proton pumps in insect epithelia.The Journal of experimental biology · 2009Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reversible assembly of the V0V1 holoenzyme from V0 and V1 subcomplexes is a widely used mechanism for regulation of vacuolar-type H+-ATPases (V-ATPases) in animal cells. In the blowfly (Calliphora vicina) salivary gland, V-ATPase is located in the apical membrane of the secretory cells and energizes the secretion of a KCl-rich saliva in response to the hormone serotonin. We have examined whether the cAMP pathway, known to be activated by serotonin, controls V-ATPase assembly and activity. Fluorescence measurements of pH changes at the luminal surface of isolated glands demonstrate that cAMP, Sp-adenosine-3',5'-cyclic monophosphorothioate, or forskolin, similar to serotonin, cause V-ATPase-dependent luminal acidification. In addition, V-ATPase-dependent ATP hydrolysis increases upon treatment with these agents. Immunofluorescence microscopy and pelleting assays have demonstrated further that V1 components become translocated from the cytoplasm to the apical membrane and V-ATPase holoenzymes are assembled at the apical membrane during conditions that increase intracellular cAMP. Because these actions occur without a change in cytosolic Ca2+, our findings suggest that the cAMP pathway mediates the reversible assembly and activation of V-ATPase molecules at the apical membrane upon hormonal stimulus.
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