ArticleProceedings of the National Academy of Sciences of the United States of America2006
Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
89 citing papers in PubMed, 173 citations in OpenAlex.
- Long-range mutual activation establishes Rho and Rac polarity during cell migration.Nature cell biology · 2026Article
- Galectin-9 regulates dendritic cell polarity and uropod contraction by modulating RhoA activity.The Journal of cell biology · 2025Article
- Competing signaling pathways controls electrotaxis.iScience · 2025Article
- The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Model supports asymmetric regulation across the intercellular junction for collective cell polarization.PLoS computational biology · 2024Article
- Excitable Rho dynamics control cell shape and motility by sequentially activating ERM proteins and actomyosin contractility.Science advances · 2024Article
- Follow the flow: Actin and membrane act as an integrated system to globally coordinate cell shape and movement.Current opinion in cell biology · 2024Review
- Cdc42 activity in the trailing edge is required for persistent directional migration of keratinocytes.Molecular biology of the cell · 2024Article
- A probabilistic algorithm for optimising the steady-state diffusional flux into a partially absorbing body.Scientific reports · 2023Article
- Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles.Nature communications · 2023Article
- Mechanosensitive mTORC2 independently coordinates leading and trailing edge polarity programs during neutrophil migration.Molecular biology of the cell · 2023Article
- Proteomic insight into human directed selection of the domesticated chicken Gallus gallus.PloS one · 2023Article
- Single-Component Optogenetic Tools for Inducible RhoA GTPase Signaling.Advanced biology · 2021Article
- Review
- Two Complementary Signaling Pathways Depict Eukaryotic Chemotaxis: A Mechanochemical Coupling Model.Frontiers in cell and developmental biology · 2021Article
- Physics of compartmentalization: How phase separation and signaling shape membrane and organelle identity.Computational and structural biotechnology journal · 2021Review
- A hybrid stochastic-deterministic mechanochemical model of cell polarization.Molecular biology of the cell · 2020Article
- Semaphorin 3F signaling actively retains neutrophils at sites of inflammation.The Journal of clinical investigation · 2020Article
- Simple Rho GTPase Dynamics Generate a Complex Regulatory Landscape Associated with Cell Shape.Biophysical journal · 2020Article
- Cellular self-organization: generating order from the abyss.Molecular biology of the cell · 2020Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Chemoattractants like fMet-Leu-Phe (fMLP) induce neutrophils to polarize with phosphatidylinositol 3,4,5-trisphosphate (PIP3) and protrusive F-actin at the front and actomyosin contraction at the sides and back. RhoA and its downstream effector, myosin II, mediate the "backness" response, which locally inhibits the "frontness" response and constrains its location to one part of the cell. In living HL-60 cells, we used a fluorescent PIP3 probe or a single-chain FRET biosensor for RhoA-GTP to assess spatial distribution of frontness or backness responses, respectively, during the first 3 min after exposure to a uniform concentration of fMLP. Increased PIP3 signal or RhoA activity initially localized randomly about the cell's periphery but progressively redistributed to the front or to the back and sides, respectively. Cells rendered unable to mount the frontness response (by inhibiting actin polymerization or Gi, a trimeric G protein) responded to a micropipette source of attractant by localizing RhoA activity at the up-gradient edge. We infer that protrusive F-actin, induced by the frontness response, constrains the spatial distribution of backness by locally reducing activation of RhoA, thereby reducing its active form at the front. Mutual incompatibility of frontness and backness is responsible for self-organization of neutrophil polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.