ArticleProceedings of the National Academy of Sciences of the United States of America2006
Transient receptor potential vanilloid 4 regulates aquaporin-5 abundance under hypotonic conditions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 110 citations in OpenAlex.
- Characterization of the effects of the vasopressin V2 receptor on sweating, fluid balance, and performance during exercise.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Trial
- 25+ Years of TRPV4: From Discovery to Translational Horizons.Research square · 2026Article
- Article
- A QUARTER CENTURY OF CALCIUM-PERMEABLE ION CHANNEL, TRPV4: PERSPECTIVES ON EXPRESSION AND FUNCTION IN ENDOTHELIAL CELLS-TIME TO TRANSLATE.Transactions of the American Clinical and Climatological Association · 2025Review
- Regulation of Neural Functions by Brain Temperature and Thermo-TRP Channels.Advances in experimental medicine and biology · 2024Review
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- AQP4-independent TRPV4 modulation of plasma membrane water permeability.Frontiers in cellular neuroscience · 2023Article
- Physiological Cooperation between Aquaporin 5 and TRPV4.International journal of molecular sciences · 2022Review
- Lens Aquaporin-5 Inserts Into Bovine Fiber Cell Plasma Membranes Via Unconventional Protein Secretion.Investigative ophthalmology & visual science · 2022Article
- Increase in the Intracellular Bulk Water Content in the Early Phase of Cell Death of Keratinocytes, Corneoptosis, as Revealed by 65 GHz Near-Field CMOS Dielectric Sensor.Molecules (Basel, Switzerland) · 2022Article
- A Repurposed Drug Screen for Compounds Regulating Aquaporin 5 Stability in Lung Epithelial Cells.Frontiers in pharmacology · 2022Article
- TRPV4 Mechanotransduction in Fibrosis.Cells · 2021Review
- The Effect of Citral on Aquaporin 5 and Trpv4 Expressions and Uterine Contraction in Rat-An Alternative Mechanism.Life (Basel, Switzerland) · 2021Article
- CaCells · 2021Review
- Hypotheses about sub-optimal hydration in the weeks before coronavirus disease (COVID-19) as a risk factor for dying from COVID-19.Medical hypotheses · 2020Article
- TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema.JCI insight · 2020Article
- Expression, Distribution and Role of Aquaporins in Various Rhinologic Conditions.International journal of molecular sciences · 2020Review
- TRPV4 activation by thermal and mechanical stimuli in disease progression.Laboratory investigation; a journal of technical methods and pathology · 2020Review
- Ions, the Movement of Water and the Apoptotic Volume Decrease.Frontiers in cell and developmental biology · 2020Review
- Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.Heliyon · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Aquaporin-5 (AQP5) is expressed in epithelia of lung, cornea, and various secretory glands, sites where extracellular osmolality is known to fluctuate. Hypertonic aquaporin (AQP) induction has been described, but little is known about the effects of a hypotonic environment on AQP abundance. We report that, when mouse lung epithelial cells were exposed to hypotonic medium, a dose-responsive decrease in AQP5 abundance was observed. Hypotonic reduction of AQP5 was blocked by ruthenium red, methanandamide, and miconazole, agents that inhibit the cation channel transient receptor potential vanilloid (TRPV) 4 present in lung epithelial cells. Several observations indicate that TRPV4 participates in hypotonic reduction of AQP5, including a requirement for extracellular calcium to achieve AQP5 reduction; an increase in intracellular calcium in mouse lung epithelial (MLE) cells after hypotonic stimulation; and reduction of AQP5 abundance after addition of the TRPV4 agonist 4alpha-Phorbol-12,13-didecanoate (4alpha-PDD). Similarly, addition of hypotonic PBS to mouse trachea in vivo decreased AQP5 within 1 h, an effect blocked by ruthenium red. To confirm a functional interaction, AQP5 was expressed in control or TRPV4-expressing human embryonic kidney (HEK) cells. Hypotonic reduction of AQP5 was observed only in the presence of TRPV4 and was blocked by ruthenium red. Combined with earlier studies, these observations indicate that AQP5 abundance is tightly regulated along a range of osmolalities and that AQP5 reduction by extracellular hypotonicity can be mediated by TRPV4. These findings have direct relevance to regulation of membrane water permeability and water homeostasis in epithelia of the lung and other organs.
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