ArticleApplied and environmental microbiology1997
Intraspecific Differentiation of Vibrio vulnificus Biotypes by Amplified Fragment Length Polymorphism and Ribotyping.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 71 citations in OpenAlex.
- Prevalence and population structure of Vibrio vulnificus on fishes from the northern Gulf of Mexico.Applied and environmental microbiology · 2012Article
- Genotypic Characterization of Vibrio vulnificus Clinical Isolates in Korea.Osong public health and research perspectives · 2011Article
- Evaluation of genotypic and phenotypic methods to distinguish clinical from environmental Vibrio vulnificus strains.Applied and environmental microbiology · 2009Article
- Emergence of a virulent clade of Vibrio vulnificus and correlation with the presence of a 33-kilobase genomic island.Applied and environmental microbiology · 2007Article
- Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and the United States.Applied and environmental microbiology · 2004Article
- Biodiversity of vibrios.Microbiology and molecular biology reviews : MMBR · 2004Review
- Intraspecific diversity of Vibrio vulnificus in Galveston Bay water and oysters as determined by randomly amplified polymorphic DNA PCR.Applied and environmental microbiology · 2003Article
- Characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting.Applied and environmental microbiology · 2000Article
- Random amplified polymorphic DNA analysis to differentiate strains of Vibrio vulnificus isolated from cockles and shrimps.The Malaysian journal of medical sciences : MJMS · 2000Article
- Amplified fragment length polymorphism fingerprinting of Pseudomonas strains from a poultry processing plant.Applied and environmental microbiology · 1999Article
- Differentiation of Vibrio alginolyticus strains isolated from Sardinian waters by ribotyping and a new rapid PCR fingerprinting method.Applied and environmental microbiology · 1999Article
- Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other vibrio species.Applied and environmental microbiology · 1999Article
- Isolation of Vibrio vulnificus serovar E from aquatic habitats in Taiwan.Applied and environmental microbiology · 1999Article
- Heterogeneity among isolates of Vibrio vulnificus recovered from eels (Anguilla anguilla) in Denmark.Applied and environmental microbiology · 1998Article
- Genetic relatedness among environmental, clinical, and diseased-eel Vibrio vulnificus isolates from different geographic regions by ribotyping and randomly amplified polymorphic DNA PCR.Applied and environmental microbiology · 1998Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intraspecific genomic relatedness of 80 Vibrio vulnificus isolates, 44 of biotype 1 and 36 of biotype 2, from different geographic origins and sources was evaluated by ribotyping and AFLP (amplified fragment length polymorphism) fingerprinting. Ribopatterns of DNAs digested with KpnI and hybridized with an oligonucleotide complementary to a highly conserved sequence in the 23S rRNA gene revealed up to 19 ribotypes in the species, which were different for the two biotypes. Sixteen different ribotypes were found within biotype 1 strains from clinical and environmental sources, and only three, recovered mainly from diseased eels, were found within biotype 2. Within this biotype, 96% of the strains showed the same ribopattern. The closest similarity was shown by the strains coming from the same eel farm, irrespectively of biotype. AFLP fingerprints obtained by selective PCR amplification of HindIII-TaqI double-restricted DNA fragments exhibited a strain-specific pattern which allowed the finest differentiation of subgroups within the eel-pathogenic isolates sharing the same ribopattern. Both techniques revealed good genetic markers for intraspecific differentiation of V. vulnificus. Ribotyping clearly separated the eel-pathogenic strains from the clinical and environmental isolates, whereas AFLP enabled the monitoring of individual strains and therefore constitutes one of the most discriminative tools for epidemiological and ecological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.