ArticleApplied and environmental microbiology1996
Growth Physiology of the Hyperthermophilic Archaeon Thermococcus litoralis: Development of a Sulfur-Free Defined Medium, Characterization of an Exopolysaccharide, and Evidence of Biofilm Formation.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 132 citations in OpenAlex.
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- Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment.Applied microbiology and biotechnology · 2022Article
- Potential functions and applications of diverse microbial exopolysaccharides in marine environments.Journal, genetic engineering & biotechnology · 2022Review
- Membrane and Extracellular Matrix Glycopolymers ofFrontiers in microbiology · 2022Article
- Exopolysaccharide composition and size inFrontiers in microbiology · 2022Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Growth Kinetics, Carbon Isotope Fractionation, and Gene Expression in the HyperthermophileApplied and environmental microbiology · 2019Article
- Insight Into Interactions of Thermoacidophilic Archaea With Elemental Sulfur: Biofilm Dynamics and EPS Analysis.Frontiers in microbiology · 2019Article
- Archaeal biofilm formation.Nature reviews. Microbiology · 2018Review
- Exopolysaccharides from Marine and Marine Extremophilic Bacteria: Structures, Properties, Ecological Roles and Applications.Marine drugs · 2018Review
- Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria.Applied and environmental microbiology · 2018Article
- Metal-tolerant thermophiles: metals as electron donors and acceptors, toxicity, tolerance and industrial applications.Environmental science and pollution research international · 2018Review
- Novel archaeal thermostable cellulases from an oil reservoir metagenome.AMB Express · 2017Article
- Identification of the ATPase Subunit of the Primary Maltose Transporter in the Hyperthermophilic Anaerobe Thermotoga maritima.Applied and environmental microbiology · 2017Article
- Contribution of Pentose Catabolism to Molecular Hydrogen Formation by Targeted Disruption of Arabinose Isomerase (araA) in the Hyperthermophilic Bacterium Thermotoga maritima.Applied and environmental microbiology · 2017Article
- Computational Exploration of Putative LuxR Solos in Archaea and Their Functional Implications in Quorum Sensing.Frontiers in microbiology · 2017Article
- Adaptive Evolution of Thermotoga maritima Reveals Plasticity of the ABC Transporter Network.Applied and environmental microbiology · 2015Article
- Use of lectins to in situ visualize glycoconjugates of extracellular polymeric substances in acidophilic archaeal biofilms.Microbial biotechnology · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nutritional characteristics of the hyperthermophilic archaeon Thermococcus litoralis have been investigated with emphasis on the development of a sulfur-free, defined growth medium, analysis of an exocellular polysaccharide, and formation of a biofilm. An artificial-seawater-based medium, containing 16 amino acids, adenine, uracil, vitamins, and trace elements, allowed T. litoralis to attain growth rates and cell densities similar to those found with complex media. Four amino acids (alanine, asparagine, glutamine, and glutamate) were not included due to their lack of effect on growth rates and cell yields. In this medium, cultures reached densities of 10(sup8) cells per ml, with doubling times of 55 min (without maltose) or 43 min (with maltose). Neither the addition of elemental sulfur nor the presence of H(inf2) significantly affected cell growth. A sparingly soluble exopolysaccharide was produced by T. litoralis grown in either defined or complex media. Analysis of the acid-hydrolyzed exopolysaccharide yielded mannose as the only monosaccharidic constituent. This exopolysaccharide is apparently involved in the formation of a biofilm on polycarbonate filters and glass slides, which is inhabited by high levels of T. litoralis. Biofilm formation by hyperthermophilic microorganisms in geothermal environments has not been examined to any extent, but further work in this area may provide information related to the interactions among high-temperature organisms.
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