ArticleApplied and environmental microbiology1996
l-Isoleucine Production with Corynebacterium glutamicum: Further Flux Increase and Limitation of Export.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- Molecular networking as a tool to annotate the metabolites of3 Biotech · 2023Article
- A synthetic synthesis to explore animal evolution and development.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with L-isoleucine in Corynebacterium glutamicum WM001.Microbial cell factories · 2018Article
- Characterization of aspartate kinase and homoserine dehydrogenase from Corynebacterium glutamicum IWJ001 and systematic investigation of L-isoleucine biosynthesis.Journal of industrial microbiology & biotechnology · 2016Article
- Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB.BMC microbiology · 2015Article
- Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis.Microbial cell factories · 2015Article
- Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes.Microbial biotechnology · 2015Article
- Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example.Frontiers in microbiology · 2015Article
- Visualization of imbalances in sulfur assimilation and synthesis of sulfur-containing amino acids at the single-cell level.Applied and environmental microbiology · 2013Article
- Effect of transport proteins on L-isoleucine production with the L-isoleucine-producing strain Corynebacterium glutamicum YILW.Journal of industrial microbiology & biotechnology · 2012Article
- Production in Escherichia coli of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with differing monomer compositions from unrelated carbon sources.Applied and environmental microbiology · 2011Article
- Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli.Microbial cell factories · 2010Article
- Pentanol isomer synthesis in engineered microorganisms.Applied microbiology and biotechnology · 2010Review
- Metabolic function of Corynebacterium glutamicum aminotransferases AlaT and AvtA and impact on L-valine production.Applied and environmental microbiology · 2008Article
- Production of 2-methyl-1-butanol in engineered Escherichia coli.Applied microbiology and biotechnology · 2008Article
- Accumulation of homolanthionine and activation of a novel pathway for isoleucine biosynthesis in Corynebacterium glutamicum McbR deletion strains.Journal of bacteriology · 2006Article
- Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources.Applied and environmental microbiology · 2005Article
- Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum.Journal of bacteriology · 2005Article
- Export of L-isoleucine from Corynebacterium glutamicum: a two-gene-encoded member of a new translocator family.Journal of bacteriology · 2002Article
- Reduction of cell lysate viscosity during processing of poly(3-hydroxyalkanoates) by chromosomal integration of the staphylococcal nuclease gene in Pseudomonas putida.Applied and environmental microbiology · 1999Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synthesis of l-isoleucine with Corynebacterium glutamicum involves 11 reaction steps, in at least five of which activity or expression is regulated. We used four genes and alleles encoding feedback-resistant enzymes (Fbr) in various combinations to assay flux increase through the sequence. During strain construction, the order of genes overexpressed was important. Only when ilvA(Fbr) was first overexpressed could hom(Fbr) be introduced. This succession apparently prevents the toxic accumulation of biosynthesis intermediates. The best strain constructed (SM13) was characterized by high-level expression of hom(Fbr), thrB, and ilvA(Fbr). With this strain a yield of 0.22 g of l-isoleucine per g of glucose was obtained, with a maximal specific productivity of 0.10 g of l-isoleucine per g (dry weight) per h. In strain SM13, with the high metabolite flux through the reaction sequence, effects on (i) other enzyme levels, (ii) time-dependent variations with process time, and (iii) concentrations of cytosolic intermediates were quantified. Most importantly, the intracellular l-isoleucine concentration is always higher at all process times than the extracellular concentration. The intracellular concentration rises to 110 mM, whereas extracellularly only 60 mM is accumulated. Also the immediate l-isoleucine precursor 2-ketomethyl valerate accumulates in the cell. Therefore, in the high-level l-isoleucine producer SM13, the export of this amino acid is the major limiting reaction step and therefore is a new target of strain design for biotechnological purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.