ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Development of microsatellite markers and analysis of intraspecific genetic variability in chickpea (Cicer arietinum L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 152 citations in OpenAlex.
- Uncovering potentials of an association panel subset for nitrogen fixation and sustainable chickpea productivity.BMC plant biology · 2025Article
- Advancing Chickpea Breeding: Omics Insights for Targeted Abiotic Stress Mitigation and Genetic Enhancement.Biochemical genetics · 2025Review
- An overview of heat stress in Chickpea (Molecular breeding : new strategies in plant improvement · 2025Review
- Identification of Insertion and Deletion (InDel) Markers for Chickpea (Plants (Basel, Switzerland) · 2024Article
- Development of SSR Markers and Evaluation of Genetic Diversity of Endangered PlantBiomolecules · 2024Article
- Disentangling potential genotypes for macro and micro nutrients and polymorphic markers in Chickpea.Scientific reports · 2023Article
- Marker trait association for biological nitrogen fixation traits in an interspecific cross of chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Genome-wide discovery of di-nucleotide SSR markers based on whole genome re-sequencing data of Cicer arietinum L. and Cicer reticulatum Ladiz.Scientific reports · 2023Article
- Breeding and Genomic Approaches towards Development of Fusarium Wilt Resistance in Chickpea.Life (Basel, Switzerland) · 2023Review
- A Comprehensive Review on Chickpea (International journal of molecular sciences · 2022Review
- Genome-wide identification and characterization of functionally relevant microsatellite markers from transcription factor genes of Tea (Camellia sinensis (L.) O. Kuntze).Scientific reports · 2022Article
- Discerning molecular diversity and association mapping for phenological, physiological and yield traits under high temperature stress in chickpea (Journal of genetics · 2021Article
- Validation of the markers linked with drought tolerance related traits for use in MAS programme in chickpea.Journal of genetics · 2021Article
- Genetic diversity and population structure of the endangered fish Pseudobagrus brevicorpus (Bagridae) using a newly developed 12-microsatellite marker.Genes & genomics · 2020Article
- Population structure and association analysis of heat stress relevant traits in chickpea (3 Biotech · 2018Article
- Genetic variation of a global germplasm collection of chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Molecular Mapping of Flowering Time Major Genes and QTLs in Chickpea (Frontiers in plant science · 2017Article
- Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height.Molecular biology reports · 2015Article
- CNMS: The preferred genic markers for comparative genomic, molecular phylogenetic, functional genetic diversity and differential gene regulatory expression analyses in chickpea.Journal of biosciences · 2015Article
- High density linkage mapping of genomic and transcriptomic SNPs for synteny analysis and anchoring the genome sequence of chickpea.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paucity of polymorphic molecular markers in chickpea (Cicer arietinum L.) has been a major limitation in the improvement of this important legume. Hence, in an attempt to develop sequence-tagged microsatellite sites (STMS) markers from chickpea, a microsatellite enriched library from the C. arietinum cv. Pusa362 nuclear genome was constructed for the identification of (CA/GT)n and (CT/GA)n microsatellite motifs. A total of 92 new microsatellites were identified, of which 74 functional STMS primer pairs were developed. These markers were validated using 9 chickpea and one C. reticulatum accession. Of the STMS markers developed, 25 polymorphic markers were used to analyze the intraspecific genetic diversity within 36 geographically diverse chickpea accessions. The 25 primer pairs amplified single loci producing a minimum of 2 and maximum of 11 alleles. A total of 159 alleles were detected with an average of 6.4 alleles per locus. The observed and expected heterozygosity values averaged 0.32 (0.08-0.91) and 0.74 (0.23-0.89) respectively. The UPGMA based dendrogram was able to distinguish all the accessions except two accessions from Afghanistan establishing that microsatellites could successfully detect intraspecific genetic diversity in chickpea. Further, cloning and sequencing of size variant alleles at two microsatellite loci revealed that the variable numbers of AG repeats in different alleles were the major source of polymorphism. Point mutations were found to occur both within and immediately upstream of the long tracts of perfect repeats, thereby bringing about a conversion of perfect motifs into imperfect or compound motifs. Such events possibly occurred in order to limit the expansion of microsatellites and also lead to the birth of new microsatellites. The microsatellite markers developed in this study will be useful for genetic diversity analysis, linkage map construction as well as for depicting intraspecific microsatellite evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.