ArticleThe Journal of cell biology2006
The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 72 citations in OpenAlex.
- Lamin B1-dependent regulation of human separase in mitosis.Nucleic acids research · 2026Article
- The Effect ofLife (Basel, Switzerland) · 2025Article
- Polyploid Formation Through Disrupting Mitotic Sister Chromatid Separation of Spermatogonia based on espl1 Heterozygous Knockout in Fish.Molecular biology and evolution · 2025Article
- Morpho-histological and Transcriptome Analysis Reveal the Unreduced Sperm Formation Mechanism in cdk1-Depletion Zebrafish.Marine biotechnology (New York, N.Y.) · 2024Article
- Role of chromosomal cohesion and separation in aneuploidy and tumorigenesis.Cellular and molecular life sciences : CMLS · 2024Review
- Building the right centriole for each cell type.The Journal of cell biology · 2018Review
- Separase prevents genomic instability by controlling replication fork speed.Nucleic acids research · 2018Article
- Cohesin Mutations in Cancer.Cold Spring Harbor perspectives in medicine · 2016Review
- Investigating the Interplay between Sister Chromatid Cohesion and Homolog Pairing in Drosophila Nuclei.PLoS genetics · 2016Article
- Cyclin B2 and p53 control proper timing of centrosome separation.Nature cell biology · 2014Article
- PP2A delays APC/C-dependent degradation of separase-associated but not free securin.The EMBO journal · 2014Article
- Article
- Multiple mechanisms contribute to centriole separation in C. elegans.Current biology : CB · 2013Article
- Sister chromatid segregation in meiosis II: deprotection through phosphorylation.Cell cycle (Georgetown, Tex.) · 2013Review
- Losing balance: the origin and impact of aneuploidy in cancer.EMBO reports · 2012Review
- Cohesin acetyltransferase Esco2 is a cell viability factor and is required for cohesion in pericentric heterochromatin.The EMBO journal · 2012Article
- Regulation of the final stage of mitosis by components of the pre-replicative complex and a polo kinase.Cell cycle (Georgetown, Tex.) · 2011Article
- Separase phosphosite mutation leads to genome instability and primordial germ cell depletion during oogenesis.PloS one · 2011Article
- Article
- Oocyte maturation failure: a syndrome of bad eggs.Fertility and sterility · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Separase is an evolutionarily conserved protease that is essential for chromosome segregation and cleaves cohesin Scc1/Rad21, which joins the sister chromatids together. Although mammalian separase also functions in chromosome segregation, our understanding of this process in mammals is still incomplete. We generated separase knockout mice, reporting an essential function for mammalian separase. Separase-deficient mouse embryonic fibroblasts exhibited severely restrained increases in cell number, polyploid chromosomes, and amplified centrosomes. Chromosome spreads demonstrated that multiple chromosomes connected to a centromeric region. Live observation demonstrated that the chromosomes of separase-deficient cells condensed, but failed to segregate, although subsequent cytokinesis and chromosome decondensation proceeded normally. These results establish that mammalian separase is essential for the separation of centromeres, but not of the arm regions of chromosomes. Other cell cycle events, such as mitotic exit, DNA replication, and centrosome duplication appear to occur normally. We also demonstrated that heterozygous separase-deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin, which is an inhibitory partner of separase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.