ArticleThe Journal of clinical investigation1991
Autocrine stimulation by erythropoietin and autonomous growth of human erythroid leukemic cells in vitro.
Article in The Journal of clinical investigation, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 55 citations in OpenAlex.
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- Erythroleukemia-historical perspectives and recent advances in diagnosis and management.Blood reviews · 2018Review
- Induction of erythropoietin increases the cell proliferation rate in a hypoxia-inducible factor-1-dependent and -independent manner in renal cell carcinoma cell lines.Oncology letters · 2013Article
- G protein-coupled receptor signalling in the cardiac nuclear membrane: evidence and possible roles in physiological and pathophysiological function.The Journal of physiology · 2012Review
- The effect of erythropoietin on normal and neoplastic cells.Biologics : targets & therapy · 2012Article
- The physiological basis of intracrine stem cell regulation.American journal of physiology. Heart and circulatory physiology · 2008Review
- Hypoxia-inducible erythropoietin signaling in squamous dysplasia and squamous cell carcinoma of the uterine cervix and its potential role in cervical carcinogenesis and tumor progression.The American journal of pathology · 2003Article
- Constitutive activation of Stat1 and Stat3 in primary erythroleukemia cells.International journal of hematology · 2002Article
- Erythroid progenitors differentiate and mature in response to endogenous erythropoietin.The Journal of clinical investigation · 2000Article
- Dominant action of mutated erythropoietin receptors on differentiation in vitro and erythroleukemia development in vivo.Oncogene · 2000Article
- Spi-1/PU.1 transgenic mice develop multistep erythroleukemias.Molecular and cellular biology · 1996Article
- Inadequate erythropoietin response to anemia: definition and clinical relevance.Annals of hematology · 1994Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autonomous colony formation is a frequent event in erythroleukemia. In 13 cases of early erythroid leukemias, we investigated whether erythropoietin (Epo) autocrine stimulation was responsible for the growth factor autonomy. Epo transcripts were detected by Northern blotting in cells from one patient. These cells also expressed an Epo receptor (1,000 receptors per cell) with a 420-pM affinity and Epo was detected in the supernatant of cultured cells. In 8 of the 13 cases, Epo transcripts were revealed by the polymerase chain reaction ranging from 0.5 to 500 copies per cell. In situ hybridization proved that these Epo transcripts were present in the blast cells. No Epo gene abnormalities were detected by Southern blotting. In two cases, leukemic cells were grown in the presence of Epo-neutralizing antibodies or Epo antisense oligomers. In one case, the antibody significantly reduced autonomous growth. In contrast, the antibody had no effect in the second case in which blast cells transcribed the Epo gene at a low level. However, Epo antisense oligomers partially inhibited autonomous growth. This inhibition was reversed by addition of exogenous Epo. Overall, these results suggest that an extracellular or intracellular autocrine Epo stimulation occurs in some cases of erythroid malignancies.
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