ArticleExtremophiles : life under extreme conditions2006
Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library.
Article in Extremophiles : life under extreme conditions, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Identification of a novel glycoside hydrolase family 8 xylanase from Deinococcus geothermalis and its application at low temperatures.Archives of microbiology · 2024Article
- Molecular and Biochemical Characterization of Xylanase Produced byJournal of microbiology and biotechnology · 2024Article
- Review
- Xylanolytic Extremozymes Retrieved From Environmental Metagenomes: Characteristics, Genetic Engineering, and Applications.Frontiers in microbiology · 2020Review
- A New Group of Modular Xylanases in Glycoside Hydrolase Family 8 from Marine Bacteria.Applied and environmental microbiology · 2018Article
- Characterization of a novel cold-active xylanase from Luteimonas species.World journal of microbiology & biotechnology · 2018Article
- The Glycoside Hydrolase Family 8 Reducing-End Xylose-Releasing Exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 Is Active on Branched Xylooligosaccharides.Applied and environmental microbiology · 2016Article
- Improved cultivation and metagenomics as new tools for bioprospecting in cold environments.Extremophiles : life under extreme conditions · 2015Review
- Kinetic and thermodynamic characterization of a novel low-temperature-active xylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis.Bioengineered · 2015Article
- Two new xylanases with different substrate specificities from the human gut bacterium Bacteroides intestinalis DSM 17393.Applied and environmental microbiology · 2014Article
- Cloning, expression and characterization of a novel cold-active and halophilic xylanase from Zunongwangia profunda.Extremophiles : life under extreme conditions · 2014Article
- Biotechnology of cold-active proteases.Biology · 2013Article
- Gene cloning, expression and characterization of a novel xylanase from the marine bacterium, Glaciecola mesophila KMM241.Marine drugs · 2013Article
- Article
- Identification and characterization of novel cellulolytic and hemicellulolytic genes and enzymes derived from German grassland soil metagenomes.Biotechnology letters · 2012Article
- Biotechnological uses of enzymes from psychrophiles.Microbial biotechnology · 2011Review
- Alkaliphilic bacteria: applications in industrial biotechnology.Journal of industrial microbiology & biotechnology · 2011Review
- Fusion of the OsmC domain from esterase EstO confers thermolability to the cold-active xylanase Xyn8 from Pseudoalteromonas arctica.Extremophiles : life under extreme conditions · 2011Article
- Crystallization and preliminary X-ray analysis of a cold-active endo-β-1,4-D-xylanase from glycoside hydrolase family 8.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Novel glycoside hydrolases identified by screening a Chinese Holstein dairy cow rumen-derived metagenome library.Applied and environmental microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a great interest in xylanases due to the wide variety of industrial applications for these enzymes. We cloned a xylanase gene (xyn8) from an environmental genomic DNA library. The encoded enzyme was predicted to be 399 amino acids with a molecular weight of 45.9 kD. The enzyme was categorized as a glycosyl hydrolase family 8 member based on sequence analysis of the putative catalytic domain. The purified enzyme was thermolabile, had an activity temperature optimum of 20 degrees C on native xylan substrate, and retained significant activity at lower temperatures. At 4 degrees C, the apparent K (m) was 3.7 mg/ml, and the apparent k (cat) was 123/s.
Indexed as
Identifiers
16532363What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.