ArticleExtremophiles : life under extreme conditions2006
Isolation and characterization of two novel ethanol-tolerant facultative-anaerobic thermophilic bacteria strains from waste compost.
Article in Extremophiles : life under extreme conditions, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Analysis of Proteome Dynamics in Early-Stage Sporulation-Inhibited Variants ofInternational journal of molecular sciences · 2025Article
- Metabolic engineering of Parageobacillus thermoglucosidasius for thermophilic production of 1-butanol.AMB Express · 2025Article
- Article
- Functional characterization of the phosphotransferase system in Parageobacillus thermoglucosidasius.Scientific reports · 2023Article
- Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 11: suitability of taxonomic units notified to EFSA until September 2019.EFSA journal. European Food Safety Authority · 2020Article
- Scientific Opinion on the update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA (2017-2019).EFSA journal. European Food Safety Authority · 2020Article
- Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genes.BMC biotechnology · 2018Article
- Effect of pH and temperature on microbial community structure and carboxylic acid yield during the acidogenic digestion of duckweed.Biotechnology for biofuels · 2018Article
- Article
- Isolation of a genetically accessible thermophilic xylan degrading bacterium from compost.Biotechnology for biofuels · 2016Article
- Creation of an ultra scale-down bioreactor mimic for rapid development of lignocellulosic enzymatic hydrolysis processes.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2015Article
- Isolation and screening of thermophilic bacilli from compost for electrotransformation and fermentation: characterization of Bacillus smithii ET 138 as a new biocatalyst.Applied and environmental microbiology · 2015Article
- Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426.Applied and environmental microbiology · 2013Article
- Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.Applied and environmental microbiology · 2012Article
- Assessment of the biomass hydrolysis potential in bacterial isolates from a volcanic environment: biosynthesis of the corresponding activities.World journal of microbiology & biotechnology · 2012Article
- Role of alcohols in growth, lipid composition, and membrane fluidity of yeasts, bacteria, and archaea.Applied and environmental microbiology · 2011Article
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9 authors.
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Abstract
In a search for potential ethanologens, waste compost was screened for ethanol-tolerant thermophilic microorganisms. Two thermophilic bacterial strains, M5EXG and M10EXG, with tolerance of 5 and 10% (v/v) ethanol, respectively, were isolated. Both isolates are facultative anaerobic, non-spore forming, non-motile, catalase-positive, oxidase-negative, Gram-negative rods that are capable of utilizing a range of carbon sources including arabinose, galactose, mannose, glucose and xylose and produce low amounts of ethanol, acetate and lactate. Growth of both isolates was observed in fully defined minimal media within the temperature range 50-80 degrees C and pH 6.0-8.0. Phylogenetic analysis of the 16S rDNA sequences revealed that both isolates clustered with members of subgroup 5 of the genus Bacillus. G+C contents and DNA-DNA relatedness of M5EXG and M10EXG revealed that they are strains belonging to Geobacillus thermoglucosidasius. However, physiological and biochemical differences were evident when isolates M5EXG and M10EXG were compared with G. thermoglucosidasius type strain (DSM 2542(T)). The new thermophilic, ethanol-tolerant strains of G. thermoglucosidasius may be candidates for ethanol production at elevated temperatures.
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