ArticlePlant physiology2006
Identification and characterization of a stress-inducible and a constitutive small heat-shock protein targeted to the matrix of plant peroxisomes.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 138 citations in OpenAlex.
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- Integrated analysis of HSP20 genes in the developing flesh of peach: identification, expression profiling, and subcellular localization.BMC plant biology · 2023Article
- Effects of Heat Stress on Plant-Nutrient Relations: An Update on Nutrient Uptake, Transport, and Assimilation.International journal of molecular sciences · 2023Review
- AGAMOUS regulates various target genes via cell cycle-coupled H3K27me3 dilution in floral meristems and stamens.The Plant cell · 2023Article
- Genome-Wide Identification of Candidate Genes Associated with Heat Stress in Mulberry (Current issues in molecular biology · 2023Article
- Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.Nature cell biology · 2023Article
- Global transcriptome profiling reveals root- and leaf-specific responses of barley (Frontiers in plant science · 2023Article
- Plant peroxisome proteostasis-establishing, renovating, and dismantling the peroxisomal proteome.Essays in biochemistry · 2022Review
- Genome-wide analysis of HSP20 gene family and expression patterns under heat stress in cucumber (Frontiers in plant science · 2022Article
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2022Article
- Diurnal dynamics of the Arabidopsis rosette proteome and phosphoproteome.Plant, cell & environment · 2021Article
- Genome-Wide Analysis of the HSP20 Gene Family and Expression Patterns ofFrontiers in genetics · 2021Article
- Article
- A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms.Scientific reports · 2020Article
- Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile AlgaFrontiers in cell and developmental biology · 2020Article
- Genome wide transcriptome analysis reveals vital role of heat responsive genes in regulatory mechanisms of lentil (Lens culinaris Medikus).Scientific reports · 2019Article
- CaHSP16.4, a small heat shock protein gene in pepper, is involved in heat and drought tolerance.Protoplasma · 2019Article
- Root proteomic and metabolic analyses reveal specific responses to drought stress in differently tolerant grapevine rootstocks.BMC plant biology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small heat-shock proteins (sHsps) are widespread molecular chaperones for which a peroxisomal localization has not yet been reported. The Arabidopsis (Arabidopsis thaliana) genome encodes two sHsps with putative peroxisomal targeting signals type 1 or 2 (PTS1 or PTS2). As demonstrated by double-labeling experiments using full-length fusion proteins with enhanced yellow fluorescent protein and deletion constructs lacking the putative targeting domains, AtHsp15.7 (At5g37670) and AtAcd31.2 (At1g06460) are targeted to the peroxisome matrix by a functional PTS1 (SKL>) and a functional PTS2 (RLx5HF), respectively. The peroxisomal localization of AtAcd31.2 was further confirmed by isolation of leaf peroxisomes from Arabidopsis by two successive sucrose density gradients, protein separation by one- and two-dimensional gel electrophoresis, and mass spectrometric protein identification. When AtHsp15.7 and AtAcd31.2 were heterologously expressed in yeast (Saccharomyces cerevisiae) and directed to the cytosol by deletion of the PTSs, both sHsps were able to complement the morphological phenotype of yeast mutants deficient in the cytosolic homologs ScHsp42 or ScHsp26. According to expression studies by reverse transcription-PCR, AtAcd31.2 is constitutively expressed, whereas AtHsp15.7 is hardly expressed under normal conditions but strongly induced by heat and oxidative stress, the latter of which was triggered by the catalase inhibitor 3-aminotriazole or the herbicide methyl viologen applied by watering of whole plants or infiltration of rosette leaves. Thus, plants are exceptional among eukaryotes in employing sHsps in the peroxisome matrix to prevent unspecific aggregation of partially denatured proteins under both physiological and stress conditions.
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