ArticleJournal of controlled release : official journal of the Controlled Release Society2006
Intramuscular delivery of DNA releasing microspheres: microsphere properties and transgene expression.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- In-drop thermal cycling of microcrystal assembly for senescence control (MASC) with minimal variation in efficacy.Advanced functional materials · 2023Article
- Development of a porous layer-by-layer microsphere with branched aliphatic hydrocarbon porogens.Nanomedicine : nanotechnology, biology, and medicine · 2023Article
- The Progress of Non-Viral Materials and Methods for Gene Delivery to Skeletal Muscle.Pharmaceutics · 2022Review
- AAVR-Displaying Interfaces: Serotype-Independent Adeno-Associated Virus Capture and Local Delivery Systems.Molecular therapy. Nucleic acids · 2019Article
- Achieving Acetylcholine Receptor Clustering in Tissue-Engineered Skeletal Muscle ConstructsFrontiers in pharmacology · 2016Article
- Strategies and hurdles using DNA vaccines to fish.Veterinary research · 2014Review
- In vivo biocompatibility of the PLGA microparticles in parotid gland.International journal of clinical and experimental pathology · 2013Article
- Gene delivery by surface immobilization of plasmid to tissue-engineering scaffolds.Gene therapy · 2010Article
- Ligand-modified gene carriers increased uptake in target cells but reduced DNA release and transfection efficiency.Nanomedicine : nanotechnology, biology, and medicine · 2010Article
- The contribution of plasmid design and release to in vivo gene expression following delivery from cationic polymer modified scaffolds.Biomaterials · 2010Article
- Drug releasing systems in cardiovascular tissue engineering.Journal of cellular and molecular medicine · 2009Review
- Polymeric Materials for Gene Delivery and DNA Vaccination.Advanced materials (Deerfield Beach, Fla.) · 2009Article
- Plasmid releasing multiple channel bridges for transgene expression after spinal cord injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Influence of particle size and antacid on release and stability of plasmid DNA from uniform PLGA microspheres.Journal of controlled release : official journal of the Controlled Release Society · 2007Article
- Precise control of PLG microsphere size provides enhanced control of drug release rate.Journal of controlled release : official journal of the Controlled Release Society · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Plasmid-loaded microspheres can provide localized and sustained release into the target tissue, and thus have the potential to enhance the efficiency of naked DNA at promoting transgene expression. In this report, microsphere design parameters are investigated by correlating the extent and duration of transgene expression intramuscularly to the polymer molecular weight and the mass of DNA delivered. Plasmid DNA was incorporated into poly (lactide-co-glycolide) microspheres using a cryogenic double emulsion process, and microspheres were injected intramuscularly. Bolus injection of naked plasmid was used for control, which exhibited transfection of muscle cells with transgene expression that gradually decreased over time. Microspheres fabricated from low molecular weight polymer had expression levels that increased from day 1 to day 92, which subsequently decreased through day 174. Decreasing the microsphere mass delivered resulted in steady expression during the same time. However, microspheres fabricated with high molecular weight polymer had expression for only 14 days. Intramuscular injection resulted in a foreign body response to the microspheres, and these infiltrating cells adjacent were primarily transfected. This understanding of microsphere properties that determine transgene expression and the distribution of transfected cells may facilitate their application to fields such as tissue engineering or DNA vaccines.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.