ArticleJournal of biotechnology2006
Methodological improvements of pyrosequencing technology.
Article in Journal of biotechnology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Next-Generation Sequencing (NGS): Platforms and Applications.Journal of pharmacy & bioallied sciences · 2024Review
- The evolution of bacterial genome assemblies - where do we need to go next?Microbiome research reports · 2022Article
- Epigenetic DNA Methylation Linked to Social Dominance.PloS one · 2015Article
- Article
- The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing.International scholarly research notices · 2014Article
- Direct oligonucleotide synthesis onto super-paramagnetic beads.Journal of biotechnology · 2013Article
- Template tailoring: Accurate determination of heterozygous alleles using peptide nucleic acid and dideoxyNTP.Electrophoresis · 2010Article
- Determination of quantitative and site-specific DNA methylation of perforin by pyrosequencing.BMC research notes · 2009Article
- Whole genome survey of coding SNPs reveals a reproducible pathway determinant of Parkinson disease.Human mutation · 2009Article
- Giant magnetoresistive biochip for DNA detection and HPV genotyping.Biosensors & bioelectronics · 2008Article
- Article
- Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications.PloS one · 2007Article
- PathogenMip assay: a multiplex pathogen detection assay.PloS one · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide polymorphism analysis and sequencing of short stretches of DNA. Although the pyrosequencing procedure is relatively straightforward, users may face challenges due to varying parameters in PCR and sequencing primer design, sample preparation and nucleotide dispensation; such challenges are labor and cost intensive. In this study, these issues have been addressed to increase signal quality and assure sequence accuracy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.