ArticlePlant cell reports2006
Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium-mediated transformation.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Removal of Transgenes and Evaluation of Yield Penalties in Genome Edited Bacterial Blight Resistant Rice Varieties.Plant biotechnology journal · 2026Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- Plant tissue culture independentJournal, genetic engineering & biotechnology · 2016Article
- Targeted modification of storage protein content resulting in improved amino acid composition of barley grain.Transgenic research · 2016Article
- High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection.Protoplasma · 2015Article
- Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight.Transgenic research · 2013Article
- The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants.Plant molecular biology · 2013Article
- Formation of complex extrachromosomal T-DNA structures in Agrobacterium tumefaciens-infected plants.Plant physiology · 2012Article
- Barley HvHMA1 is a heavy metal pump involved in mobilizing organellar Zn and Cu and plays a role in metal loading into grains.PloS one · 2012Article
- Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato.Molecular biotechnology · 2011Article
- Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA.Plant cell reports · 2010Article
- Generation of marker-free Bt transgenic indica rice and evaluation of its yellow stem borer resistance.Journal of applied genetics · 2010Article
- Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize.International journal of plant genomics · 2009Article
- Co-integration, co-expression and inheritance of unlinked minimal transgene expression cassettes in an apomictic turf and forage grass (Paspalum notatum Flugge).Plant cell reports · 2008Article
- Transgene integration and organization in cotton (Gossypium hirsutum L.) genome.Transgenic research · 2008Article
- Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules.Plant cell reports · 2006Article
- Insights into recognition of the T-DNA border repeats as termination sites for T-strand synthesis by Agrobacterium tumefaciens.Transgenic research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a large-scale study on the frequency of transgene and T-DNA backbone integration following Agrobacterium-mediated transformation of immature barley embryos. One hundred and ninety-one plant lines were regenerated after hygromycin selection and visual selection for GFP expression at the callus stage. Southern blotting performed on a subset of 53 lines that were PCR positive for the GFP gene documented the integration of the GFP gene in 27 of the lines. Twenty-three of these lines expressed GFP in T(1) plantlets. Southern blotting with a vector backbone probe revealed that 13 of the 27 lines possessed one or more vector backbone fragments illustrating the regular occurrence of vector backbone integration following Agrobacterium infection of barley immature embryos.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.