ArticleNucleic acids research2006
The Rhizobium etli sigma70 (SigA) factor recognizes a lax consensus promoter.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Genomic engineering inFrontiers in microbiology · 2025Article
- RhizoBindingSites v2.0 Is a Bioinformatic Database of DNA Motifs Potentially Involved in Transcriptional Regulation Deduced From Their Genomic Sites.Bioinformatics and biology insights · 2024Article
- Highly Efficient CRISPR-Mediated Base Editing inFrontiers in microbiology · 2021Article
- Genome and sequence determinants governing the expression of horizontally acquired DNA in bacteria.The ISME journal · 2020Article
- Control of nitrogen fixation in bacteria that associate with cereals.Nature microbiology · 2020Article
- RhizoBindingSites, a Database of DNA-Binding Motifs in Nitrogen-Fixing Bacteria Inferred Using a Footprint Discovery Approach.Frontiers in microbiology · 2020Article
- Expression of the arsenite oxidation regulatory operon in Rhizobium sp. str. NT-26 is under the control of two promoters that respond to different environmental cues.MicrobiologyOpen · 2018Article
- RNAseq analysis of α-proteobacterium Gluconobacter oxydans 621H.BMC genomics · 2018Article
- The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2).Nature communications · 2016Article
- Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli.Frontiers in microbiology · 2016Article
- Evolution of a Sigma Factor: An All-In-One of Gene Duplication, Horizontal Gene Transfer, Purifying Selection, and Promoter Differentiation.Frontiers in microbiology · 2016Article
- RNA-Seq analysis of the multipartite genome of Rhizobium etli CE3 shows different replicon contributions under heat and saline shock.BMC genomics · 2014Article
- Transcription of Ehrlichia chaffeensis genes is accomplished by RNA polymerase holoenzyme containing either sigma 32 or sigma 70.PloS one · 2013Article
- Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3.Virology journal · 2012Article
- Activity of the Rhodopseudomonas palustris p-coumaroyl-homoserine lactone-responsive transcription factor RpaR.Journal of bacteriology · 2011Article
- The BlcC (AttM) lactonase of Agrobacterium tumefaciens does not quench the quorum-sensing system that regulates Ti plasmid conjugative transfer.Journal of bacteriology · 2009Article
- Dual promoters control expression of the Bacillus anthracis virulence factor AtxA.Journal of bacteriology · 2008Article
- Transcriptional interference and repression modulate the conjugative ability of the symbiotic plasmid of Rhizobium etli.Journal of bacteriology · 2008Article
- The alternative sigma factor sigmaH is required for toxin gene expression by Bacillus anthracis.Journal of bacteriology · 2007Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A collection of Rhizobium etli promoters was isolated from a genomic DNA library constructed in the promoter-trap vector pBBMCS53, by their ability to drive the expression of a gusA reporter gene. Thirty-seven clones were selected, and their transcriptional start-sites were determined. The upstream sequence of these 37 start-sites, and the sequences of seven previously identified promoters were compared. On the basis of sequence conservation and mutational analysis, a consensus sequence CTTGACN16-23TATNNT was obtained. In this consensus sequence, nine on of twelve bases are identical to the canonical Escherichia coli sigma70 promoter, however the R.etli promoters only contain 6.4 conserved bases on average. We show that the R.etli sigma factor SigA recognizes all R.etli promoters studied in this work, and that E.coli RpoD is incapable of recognizing them. The comparison of the predicted structure of SigA with the known structure of RpoD indicated that regions 2.4 and 4.2, responsible for promoter recognition, are different only by a single amino acid, whereas the region 1 of SigA contains 72 extra residues, suggesting that the differences contained in this region could be related to the lax promoter recognition of SigA.
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