ArticleThe Journal of physiology2006
Dynamic behaviour of half-sarcomeres during and after stretch in activated rabbit psoas myofibrils: sarcomere asymmetry but no 'sarcomere popping'.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 98 citations in OpenAlex.
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- Levosimendan's Effects on Length-Dependent Activation in Murine Fast-Twitch Skeletal Muscle.International journal of molecular sciences · 2024Article
- Characterizing residual and passive force enhancements in cardiac myofibrils.Biophysical journal · 2023Article
- Effects of Hydrostatic-Pressure on Muscle Contraction: A Look Back on Some Experimental Findings.International journal of molecular sciences · 2023Review
- Numerical instability of Hill-type muscle models.Journal of the Royal Society, Interface · 2023Review
- Contractile State Dependent Sarcomere Length Variability in Isolated Guinea-Pig Cardiomyocytes.Frontiers in physiology · 2022Article
- Effect of Active Lengthening and Shortening on Small-Angle X-ray Reflections in Skinned Skeletal Muscle Fibres.International journal of molecular sciences · 2021Article
- Single sarcomere contraction dynamics in a whole muscle.Scientific reports · 2018Article
- Basic science and clinical use of eccentric contractions: History and uncertainties.Journal of sport and health science · 2018Review
- Titin-mediated thick filament activation stabilizes myofibrils on the descending limb of their force-length relationship.Journal of sport and health science · 2018Article
- A continuum-mechanical skeletal muscle model including actin-titin interaction predicts stable contractions on the descending limb of the force-length relation.PLoS computational biology · 2017Article
- Microfluidic perfusion shows intersarcomere dynamics within single skeletal muscle myofibrils.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Physiological Mechanisms of Eccentric Contraction and Its Applications: A Role for the Giant Titin Protein.Frontiers in physiology · 2017Review
- The role of sarcomere length non-uniformities in residual force enhancement of skeletal muscle myofibrils.Royal Society open science · 2016Article
- Poorly understood aspects of striated muscle contraction.BioMed research international · 2015Review
- Phosphorylation of myosin regulatory light chain has minimal effect on kinetics and distribution of orientations of cross bridges of rabbit skeletal muscle.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Sarcomere lengths in human extensor carpi radialis brevis measured by microendoscopy.Muscle & nerve · 2013Article
- Force enhancement in lengthening contractions of cat soleus muscle in situ: transient and steady-state aspects.Physiological reports · 2013Article
- Eccentric exercise training: modalities, applications and perspectives.Sports medicine (Auckland, N.Z.) · 2013Article
- Reply from K. A. P. Edman.The Journal of physiology · 2013Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
We examined length changes of individual half-sarcomeres during and after stretch in actively contracting, single rabbit psoas myofibrils containing 10-30 sarcomeres. The myofibrils were fluorescently immunostained so that both Z-lines and M-bands of sarcomeres could be monitored by video microscopy simultaneously with the force measurement. Half-sarcomere lengths were determined by processing of video images and tracking the fluorescent Z-line and M-band signals. Upon Ca2+ activation, during the rise in force, active half-sarcomeres predominantly shorten but to different extents so that an active myofibril consists of half-sarcomeres of different lengths and thus asymmetric sarcomeres, i.e. shifted A-bands, indicating different amounts of filament overlap in the two halves. When force reached a plateau, the myofibril was stretched by 15-20% resting length (L0) at a velocity of approximately 0.2 L0 s(-1). The myofibril force response to a ramp stretch is similar to that reported from muscle fibres. Despite the approximately 2.5-fold increase in force due to the stretch, the variability in half-sarcomere length remained almost constant during the stretch and A-band shifts did not progress further, independent of whether half-sarcomeres shortened or lengthened during the initial Ca2+ activation. Moreover, albeit half-sarcomeres lengthened to different extents during a stretch, rapid elongation of individual sarcomeres beyond filament overlap ('popping') was not observed. Thus, in contrast to predictions of the 'popping sarcomere' hypothesis, a stretch rather stabilizes the uniformity of half-sarcomere lengths and sarcomere symmetry. In general, the half-sarcomere length changes (dynamics) before and after stretch were slow and the dynamics after stretch were not readily predictable on the basis of the steady-state force-sarcomere length relation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.