ArticleCurrent biology : CB2006
Molecular requirements for kinetochore-associated microtubule formation in mammalian cells.
Article in Current biology : CB, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
124 citing papers in PubMed, 222 citations in OpenAlex.
- The Chromatin Protein CFDP1 Activates TPX2 and Promotes Chromosomal Microtubule Nucleation and Spindle Assembly.International journal of molecular sciences · 2026Article
- The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Bayesian data driven modelling of kinetochore dynamics: Space-time organisation of the human metaphase plate.PLoS computational biology · 2026Article
- The role of chromosome/kinetochore-driven microtubule generation in Drosophila spindle assembly.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Branching, crosslinking, and decentralization of microtubules accelerates intracellular assembly.Biophysical journal · 2025Article
- Diverse microtubule-binding repeats regulate TPX2 activities at distinct locations within the spindle.The Journal of cell biology · 2025Article
- ARHGEF17/TEM4 regulates the cell cycle through control of G1 progression.The Journal of cell biology · 2025Article
- Overexpression of the TPX2 gene is associated with enhanced tumor malignancy in lung adenocarcinoma and identification of marine natural inhibitors of the Aurora kinase A-TPX2 protein complex.In silico pharmacology · 2025Article
- HURP regulates Kif18A recruitment and activity to synergistically control microtubule dynamics.Nature communications · 2024Article
- Antiparallel microtubule bundling supports KIF15-driven mitotic spindle assembly.Molecular biology of the cell · 2024Article
- Molecular interplay between HURP and Kif18A in mitotic spindle regulation.Research square · 2024Article
- CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation.PLoS biology · 2024Article
- Changes in spindle morphology driven by TPX2 overexpression in MYC-driven breast cancer cells.microPublication biology · 2024Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression.Current biology : CB · 2023Article
- A nucleotide binding-independent role for γ-tubulin in microtubule capping and cell division.The Journal of cell biology · 2023Article
- Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers.eLife · 2022Article
- Article
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells.Current biology : CB · 2022Article
64 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In centrosome-containing cells, microtubules nucleated at centrosomes are thought to play a major role in spindle assembly. In addition, microtubule formation at kinetochores has also been observed, most recently under physiological conditions in live cells. The relative contributions of microtubule formation at kinetochores and centrosomes to spindle assembly, and their molecular requirements, remain incompletely understood. Using mammalian cells released from nocodazole-induced disassembly, we observed microtubule formation at centrosomes and at Bub1-positive sites on chromosomes. Kinetochore-associated microtubules rapidly coalesced into pole-like structures in a dynein-dependent manner. Microinjection of excess importin-beta or depletion of the Ran-dependent spindle assembly factor, TPX2, blocked kinetochore-associated microtubule formation, enhanced centrosome-associated microtubule formation, but did not prevent chromosome capture by centrosomal microtubules. Depletion of the chromosome passenger protein, survivin, reduced microtubule formation at kinetochores in an MCAK-dependent manner. Microtubule formation in cells depleted of Bub1 or Nuf2 was indistinguishable from that in controls. Our data demonstrate that microtubule assembly at centrosomes and kinetochores is kinetically distinct and differentially regulated. The presence of microtubules at kinetochores provides a mechanism to reconcile the time required for spindle assembly in vivo with that observed in computer simulations of search and capture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.